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iFluor® 405 azide

iFluor® 405 azide is reasonably stable and shows good reactivity and selectivity with the alkyne group in click chemistry. iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 405 dyes have fluorescence excitation and emission maxima of ~403 nm and ~427 nm respectively. The iFluor® 405 family has spectral properties essentially identical to those of Alexa Fluor® 405 (Alexa Fluor® 405 is the trademark of ThermoFisher). Its fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a great alternative to Alexa Fluor® 405.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 647 azide65667025000010.2510.030.03
iFluor® 488 azide4915167500010.910.210.11
iFluor® 555 azide55757010000010.6410.230.14
iFluor® 405 Styramide4034273700010.9110.480.77
iFluor® 790 Azide78781225000010.1310.10.09

Citations

View all 1 citations: Citation Explorer
PF-D-Trimer, a protective SARS-CoV-2 subunit vaccine: immunogenicity and application
Authors: Zhang, Zhihao and Zhou, Jinhu and Ni, Peng and Hu, Bing and Jolicoeur, Normand and Deng, Shuang and Xiao, Qian and He, Qian and Li, Gai and Xia, Yan and others,
Journal: npj Vaccines (2023): 38

References

View all 5 references: Citation Explorer
Effects of Viscosity and Refractive Index on the Emission and Diffusion Properties of Alexa Fluor 405 Using Fluorescence Correlation and Lifetime Spectroscopies.
Authors: van Zanten, Camila and Melnikau, Dzmitry and Ryder, Alan G
Journal: Journal of fluorescence (2021): 835-845
Egg autofluorescence and options for detecting peanut agglutinin binding for the identification of Haemonchus contortus eggs in fecal samples.
Authors: Abbas, Ibrahim and Hildreth, Michael
Journal: Veterinary parasitology (2019): 69-74
Identification of novel cell-impermeant fluorescent substrates for testing the function and drug interaction of Organic Anion-Transporting Polypeptides, OATP1B1/1B3 and 2B1.
Authors: Patik, Izabel and Székely, Virág and Német, Orsolya and Szepesi, Áron and Kucsma, Nóra and Várady, György and Szakács, Gergely and Bakos, Éva and Özvegy-Laczka, Csilla
Journal: Scientific reports (2018): 2630
Energy transfer between a biological labelling dye and gold nanorods.
Authors: Racknor, Chris and Singh, Mahi R and Zhang, Yinan and Birch, David J S and Chen, Yu
Journal: Methods and applications in fluorescence (2013): 015002
Violet laser diodes in flow cytometry: an update.
Authors: Telford, William and Kapoor, Veena and Jackson, James and Burgess, Walter and Buller, Gayle and Hawley, Teresa and Hawley, Robert
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2006): 1153-60
Page updated on November 21, 2024

Ordering information

Price
Unit size
Catalog Number1094
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

660.64

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.48

Correction Factor (280 nm)

0.77

Extinction coefficient (cm -1 M -1)

370001

Excitation (nm)

403

Emission (nm)

427

Quantum yield

0.911

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
iFluor® 405 azide is reasonably stable and shows good reactivity and selectivity with alkyne group in click chemistry. iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm).
iFluor® 405 azide is reasonably stable and shows good reactivity and selectivity with alkyne group in click chemistry. iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm).
iFluor® 405 azide is reasonably stable and shows good reactivity and selectivity with alkyne group in click chemistry. iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm).

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Click Chemistry
iFluor® Dyes and Kits

Alternative formats

iFluor® 405 succinimidyl ester
iFluor® 405 amine
iFluor® 405 maleimide
iFluor® 405 acid
iFluor® 405 hydrazide
iFluor® A7 SE
iFluor® 350 maleimide
iFluor® 488 maleimide