XFD514 alkyne

• Ex/Em: 518/543 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Reactive Group: Alkyne
• Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
• Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
• Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally identical to Alexa Fluor™ 514 (ThermoFisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4–10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays. It also serves as an effective replacement for rhodamine 6G in applications requiring enhanced performance.

The alkyne derivative of XFD514 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.