XFD568 TCO
Product key features
- Ex/Em: 579/603 nm
- Extinction coefficient: 91,300 cm-1M-1
- Reactive Group: TCO
- Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
- Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
- Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
Product description
XFD568, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 568 (ThermoFisher). This bright orange-fluorescent dye is efficiently excited by the 568 nm line of the AR-Kr mixed-gas laser and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD568 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced imaging techniques like dSTORM. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD568 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.
XFD568 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 579 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
XFD488 TCO | 499 | 520 | 73000 | 0.921 | 0.3 | 0.11 |
XFD568 amine | 579 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
XFD350 TCO | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD405 TCO | 401 | 421 | 35,000 | - | 0.23 | 0.70 |
XFD430 TCO | 432 | 540 | 15,000 | - | - | 0.28 |
XFD514 TCO | 518 | 543 | 80000 | - | 0.31 | 0.18 |
XFD546 TCO | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
XFD532 TCO | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
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