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AAT Bioquest

XFD568 TCO

Product key features

  • Ex/Em: 579/603 nm
  • Extinction coefficient: 91,300 cm-1M-1
  • Reactive Group: TCO
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
  • Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD568, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 568 (ThermoFisher). This bright orange-fluorescent dye is efficiently excited by the 568 nm line of the AR-Kr mixed-gas laser and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD568 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced imaging techniques like dSTORM. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD568 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

XFD568 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.450.46
XFD488 TCO499520730000.9210.30.11
XFD568 amine579603913000.6910.450.46
XFD350 TCO34344119000-0.250.19
XFD405 TCO40142135,000-0.230.70
XFD430 TCO43254015,000--0.28
XFD514 TCO51854380000-0.310.18
XFD546 TCO5615721120000.7910.210.12
XFD532 TCO534553810000.6110.240.09
XFD568 azide579603913000.6910.450.46
XFD568 alkyne579603913000.6910.450.46
XFD568 Tetrazine579603913000.6910.450.46
XFD594 TCO590618900000.6610.430.56
XFD610 TCO611629144,000-0.430.44
XFD633 TCO632650159,000--0.55
XFD660 TCO663691132000-0.000.10
Cy3B TCO56057112000010.5810.0480.069
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Page updated on December 17, 2024

Ordering information

Price
Unit size
Catalog Number70136
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

903.03

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.45

Correction Factor (280 nm)

0.46

Extinction coefficient (cm -1 M -1)

91300

Excitation (nm)

579

Emission (nm)

603

Quantum yield

0.691

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501