Cy3B TCO

Name: Cy3B TCO
Brand: AAT Bioquest
SKU: 946
Price: 421 USD
Availability: InStock

Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B TCO can be used for conjugations with tetrazine-containing biological molecules under copper-free conditions. Compared to the other click reactions, the TCO-tetrazine reaction gives much higher yields under mild reaction conditions. Cy3® is the trademark of GE Healthcare.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Cy3B acid	560	571	120000¹	0.58¹	0.048	0.069
Cy3B maleimide	560	571	120000¹	0.58¹	0.048	0.069
Cy3B azide	560	571	120000¹	0.58¹	0.048	0.069
Cy3B alkyne	560	571	120000¹	0.58¹	0.048	0.069
Cy3B DBCO	560	571	120000¹	0.58¹	0.048	0.069
Cy3B Tetrazine	560	571	120000¹	0.58¹	0.048	0.069
XFD488 TCO	499	520	73000	0.92¹	0.3	0.11
XFD350 TCO	343	441	19000	-	0.25	0.19
XFD405 TCO	401	421	35,000	-	0.23	0.70
XFD430 TCO	432	540	15,000	-	-	0.28
XFD514 TCO	518	543	80000	-	0.31	0.18
XFD546 TCO	561	572	112000	0.79¹	0.21	0.12
XFD532 TCO	534	553	81000	0.61¹	0.24	0.09
XFD568 TCO	579	603	91300	0.69¹	0.45	0.46
XFD594 TCO	590	618	90000	0.66¹	0.43	0.56
XFD610 TCO	611	629	144,000	-	0.43	0.44
XFD633 TCO	632	650	159,000	-	-	0.55
XFD660 TCO	663	691	132000	-	0.00	0.10
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References

View all 3 references: Citation Explorer

Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)

Clickable Methyltetrazine-Indocarbocyanine Lipids: A Multicolor Tool Kit for Efficient Modifications of Cell Membranes.
Authors: Gaikwad, Hanmant and Wang, Guankui and Smith, Weston J and Alexander, Keisha L and D'Alessandro, Angelo and Zhang, Wei and Purev, Enkhtsetseg and Simberg, Dmitri
Journal: Bioconjugate chemistry (2019): 2106-2114

Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8

Page updated on December 17, 2024

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Catalog Number	946
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Physical properties

Molecular weight	1283.58
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.048
Correction Factor (280 nm)	0.069
Extinction coefficient (cm ^-1 M ^-1)	120000¹
Excitation (nm)	560
Emission (nm)	571
Quantum yield	0.58¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501