iFluor® 680-Wheat Germ Agglutinin (WGA) Conjugate
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
Correction Factor (260 nm) | 0.097 |
Correction Factor (280 nm) | 0.094 |
Extinction coefficient (cm -1 M -1) | 2200001 |
Excitation (nm) | 684 |
Emission (nm) | 701 |
Quantum yield | 0.231 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
Correction Factor (260 nm) 0.097 | Correction Factor (280 nm) 0.094 | Extinction coefficient (cm -1 M -1) 2200001 | Excitation (nm) 684 | Emission (nm) 701 | Quantum yield 0.231 |
Platform
Fluorescence microscope
Excitation | Cy5 filter set |
Emission | Cy5 filter set |
Recommended plate | Black wall/clear bottom |
Example protocol
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 500 µL of ddH2O into the powder form to make a 2 mg/mL stock solution.
Note: The reconstituted conjugate solution can be stored at 2-8 °C for short-term storage or at -20 °C for long-term storage.
PREPARATION OF WORKING SOLUTION
Add 5 µL of 200X WGA conjugate solution to 1 mL HHBS Buffer.
Note: The optimized staining concentration may be different with different cell lines. The recommended starting concentration is 5-10 µg/mL for live cells.
SAMPLE EXPERIMENTAL PROTOCOL
Warm the vial to room temperature centrifuge briefly before opening. Staining protocols vary with applications. Appropriate dilution of conjugates should be determined experimentally.
- Wash cells twice with a HHBS buffer.
Add 100 µL iFluor® 680-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at 37 °C.
- Wash cells twice with HHBS buffer.
Image cells on a fluorescence microscope using Cy5 filter set.
WGA conjugates can be also used to stain fixed cells.
Fix cells with 4% Formaldehyde in PBS.
Note: For fixed cell membrane staining, it is recommended to stain without the permeabilization step. A permeabilization step after fixation can facilitate staining intracellular compartments such as Golgi and Endoplasmic Reticulum (ER) structures.
Add 100 µL iFluor® 680-WGA working solution.
- Incubate cells with WGA working solution for 10-30 minutes at room temperature.
- Wash cells twice with HHBS buffer.
Image cells on a fluorescence microscope using Cy5 filter set.
Spectrum
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fifluor_680.png&w=2048&q=50)
Spectral properties
Correction Factor (260 nm) | 0.097 |
Correction Factor (280 nm) | 0.094 |
Extinction coefficient (cm -1 M -1) | 2200001 |
Excitation (nm) | 684 |
Emission (nm) | 701 |
Quantum yield | 0.231 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 488-Wheat Germ Agglutinin (WGA) Conjugate | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555-Wheat Germ Agglutinin (WGA) Conjugate | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594-Wheat Germ Agglutinin (WGA) Conjugate | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
iFluor® 647-Wheat Germ Agglutinin (WGA) Conjugate | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 350-Wheat Germ Agglutinin (WGA) Conjugate | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 532-Wheat Germ Agglutinin (WGA) Conjugate | 537 | 560 | 900001 | 0.681 | 0.26 | 0.16 |
iFluor® 700-Wheat Germ Agglutinin (WGA) Conjugate | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate | 757 | 779 | 2750001 | 0.121 | 0.044 | 0.039 |
iFluor® 790-Wheat Germ Agglutinin (WGA) Conjugate | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
Images
References
Authors: Zeden, Merve S and Gallagher, Laura A and Bueno, Emilio and Nolan, Aaron C and Ahn, Jongsam and Shinde, Dhananjay and Razvi, Fareha and Sladek, Margaret and Burke, Órla and O'Neill, Eoghan and Fey, Paul D and Cava, Felipe and Thomas, Vinai C and O'Gara, James P
Journal: bioRxiv : the preprint server for biology (2023)
Authors: Singh, Anamika and Arango, Juan C and Shi, Anni and d'Aliberti, Joseph B and Claridge, Shelley A
Journal: Journal of the American Chemical Society (2023): 1668-1677
Authors: Klawonn, Isabell and Dunker, Susanne and Kagami, Maiko and Grossart, Hans-Peter and Van den Wyngaert, Silke
Journal: Microbial ecology (2023): 9-23
Authors: Zhao, Zhenyi and Lv, Jian and Guo, Ningning and Guo, Qiuxiao and Zeng, Sai and Fang, Yu and Chen, Weixin and Wang, Zhihua
Journal: Current drug targets (2023): 371-378
Authors: Skoll, Katharina and Palmetzhofer, Julia and Lummerstorfer, Maria and Anzengruber, Maria and Gabor, Franz and Wirth, Michael
Journal: Nanomedicine : nanotechnology, biology, and medicine (2023): 102685
Authors: Adachi, Ryota and Matsushita, Takahiko and Koyama, Tetsuo and Hatano, Ken and Matsuoka, Koji
Journal: Bioorganic & medicinal chemistry (2023): 117209
Authors: Fu, Hui and Kong, Bin and Zhu, Jun and Huang, He and Shuai, Wei
Journal: International immunopharmacology (2023): 109795
Authors: Deng, Zhilan and Sun, Kaixin and Sha, Dongyu and Zhang, Yinuo and Guo, Jiaxin and Yan, Guiqin and Zhang, Weichen and Liu, Ming and Deng, Xiaoyan and Kang, Hongyan and Sun, Anqiang
Journal: Atherosclerosis (2023): 24-33
Authors: Babot, Jaime D and Argañaraz-Martínez, Eloy and Apella, María C and Perez Chaia, Adriana
Journal: Food and bioprocess technology (2023): 1478-1487
Authors: Taghavi, Sharven and Abdullah, Sarah and Shaheen, Farhana and Packer, Jacob and Duchesne, Juan and Braun, Stephen E and Steele, Chad and Pociask, Derek and Kolls, Jay and Jackson-Weaver, Olan
Journal: Shock (Augusta, Ga.) (2023)
Application notes
FAQ
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?