Wheat Germ Agglutinin (WGA)

mFluor™ Violet 540-Wheat Germ Agglutinin (WGA) Conjugate

Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 β-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The mFluor™ Violet 540 labeled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 535 nm. Notably, the mFluor™ Violet 540 WGA conjugate retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis of various scientific investigations.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25454	1 mg	Price

References

View all 50 references: Citation Explorer

Application of immuno- and affinity labeling with fluorescent dyes to in-resin CLEM of Epon-embedded cells.

Authors:

Tanida, Isei and Yamaguchi, Junji and Suzuki, Chigure and Kakuta, Soichiro and Uchiyama, Yasuo

Journal:

Heliyon (2023): e17394

Intercomparison of Two Fluorescent Dyes to Visualize Parasitic Fungi (Chytridiomycota) on Phytoplankton.

Authors:

Klawonn, Isabell and Dunker, Susanne and Kagami, Maiko and Grossart, Hans-Peter and Van den Wyngaert, Silke

Journal:

Microbial ecology (2023): 9-23

Supramolecular Binding with Lectins: A New Route for Non-Covalent Functionalization of Polysaccharide Matrices.

Authors:

Montroni, Devis and Di Giosia, Matteo and Calvaresi, Matteo and Falini, Giuseppe

Journal:

Molecules (Basel, Switzerland) (2022)

Fluorescence background quenching as a means to increase Signal to Background ratio - a proof of concept during Nerve Imaging.

Authors:

Buckle, Tessa and van der Wal, Steffen and van Willigen, Danny M and Aalderink, Germaine and KleinJan, Gijs H and van Leeuwen, Fijs W B

Journal:

Theranostics (2020): 9890-9898

Cell Envelope Integrity and Capsule Characterization of Rhodotorula mucilaginosa Strains from Clinical and Environmental Sources.

Authors:

Yockey, Johnathan and Andres, Luke and Carson, Moleigh and Ory, Jeramia J and Reese, Amy J

Journal:

mSphere (2019)

Physical properties

Solvent

Water

Spectral properties

Absorbance (nm)	401
Correction factor (260 nm)	1.326
Correction factor (280 nm)	0.543
Extinction coefficient (cm ^-1 M ^-1)	18000 ¹
Excitation (nm)	402
Emission (nm)	535
Quantum yield	0.21 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	402 nm
Emission	535 nm
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 8, 2024