Wheat Germ Agglutinin (WGA)

XFD532 Same Structure to Alexa Fluor® 532

XFD532, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 532, a trademark of ThermoFisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives, and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD532 WGA conjugate, like its counterpart Alexa Fluor® 532 WGA conjugate, emits a bright yellow fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Wheat Germ Agglutinin, XFD488 Labeled XFD488 Same Structure to Alexa Fluor™ 488	499	520	71000	0.92¹	0.30	0.11
Wheat Germ Agglutinin, XFD594 Labeled XFD594 Same Structure to Alexa Fluor™ 594	590	618	90000	0.66¹	0.43	0.56
Wheat Germ Agglutinin, XFD350 Labeled XFD350 Same Structure to Alexa Fluor® 350	343	441	19000	-	0.25	0.19
Wheat Germ Agglutinin, XFD555 Labeled XFD555 Same Structure to Alexa Fluor® 555	553	568	150000	0.1¹	0.08	0.08
Wheat Germ Agglutinin, XFD647 Labeled XFD647 Same Structure to Alexa Fluor® 647	650	671	239000	0.33¹	0.00	0.03
Wheat Germ Agglutinin, XFD750 Labeled XFD750 Same Structure to Alexa Fluor® 750	752	776	240000	0.12¹	0.00	0.04

References

View all 4 references: Citation Explorer

Fluorescence quenching of tryptophan by trifluoroacetamide.
Authors: Midoux, P and Wahl, P and Auchet, J C and Monsigny, M
Journal: Biochimica et biophysica acta (1984): 16-25

The binding of monosaccharides to wheat germ agglutinin: fluorescence and NMR investigations.
Authors: Midoux, P and Grivet, J P and Delmotte, F and Monsigny, M
Journal: Biochemical and biophysical research communications (1984): 603-11

Localization of chitin in the cell wall of Candida albicans by means of wheat germ agglutinin. Fluorescence and ultrastructural studies.
Authors: Tronchin, G and Poulain, D and Herbaut, J and Biguet, J
Journal: European journal of cell biology (1981): 121-8

Nanosecond-pulse fluorimetry of wheat-germ agglutinin (lectin).
Authors: Privat, J P and Wahl, P and Monsigny, M and Auchet, J C
Journal: European journal of biochemistry (1976): 573-80

Page updated on October 8, 2024

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Physical properties

Solvent

Water

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	81000
Excitation (nm)	534
Emission (nm)	553
Quantum yield	0.61¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501