Wheat Germ Agglutinin (WGA)

XFD647, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 647, a trademark of ThermoFisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives and conjugates are widely used to label yeast bud scars, fibrotic scar tissue, and the cell membrane of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD647 WGA conjugate, like its counterpart Alexa Fluor® 647 WGA conjugate, emits a bright red fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).

Page updated on October 8, 2024