iFluor® Ultra 750 maleimide

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1208.27
Solvent	DMSO

Spectral properties

Absorbance (nm)	750
Correction Factor (260 nm)	0.04
Correction Factor (280 nm)	0.05
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	749
Emission (nm)	773
Quantum yield	0.32¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

Overview SDS Protocol

Molecular weight

1208.27

Absorbance (nm)

750

Correction Factor (260 nm)

0.04

Correction Factor (280 nm)

0.05

Extinction coefficient (cm ^-1 M ^-1)

250000¹

Excitation (nm)

749

Emission (nm)

773

Quantum yield

0.32¹

The iFluor® Ultra series represents an enhancement of our established iFluor® dyes, optimized for antibody labeling in fluorescence imaging and flow cytometry. Within this series, iFluor® Ultra 750 is a bright NIR fluorescent dye excitable by 633 nm to 685 nm laser lines, with a peak emission at 773 nm. Antibody conjugates with iFluor® Ultra 750 demonstrate superior brightness compared to those labeled with spectrally similar dyes such as Cy7, Dylight 755, IRDye750, and Alexa Fluor® 750 under the same conditions. Additionally, iFluor® Ultra 750 maintains stable fluorescence across a pH range of 4 to 10. The maleimide derivative of iFluor® Ultra 750 is widely used for conjugation to thiol groups on proteins, oligonucleotide thiophosphates, or low molecular weight ligands. Conjugates with iFluor® Ultra 750 demonstrate higher fluorescence intensity and greater photostability compared to those with other spectrally similar fluorophores, enhancing their utility for advanced fluorescence applications. Fluorescent dye-conjugated antibodies are crucial for protein identification in various applications, including fluorescent cell imaging, flow cytometry, western blotting, and immunohistochemistry. The advantages of these conjugates include increased sensitivity, multiplexing capabilities, and ease of use, facilitating complex biological studies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® Ultra 750 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	82.763 µL	413.815 µL	827.63 µL	4.138 mL	8.276 mL
5 mM	16.553 µL	82.763 µL	165.526 µL	827.63 µL	1.655 mL
10 mM	8.276 µL	41.381 µL	82.763 µL	413.815 µL	827.63 µL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)	750
Correction Factor (260 nm)	0.04
Correction Factor (280 nm)	0.05
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	749
Emission (nm)	773
Quantum yield	0.32¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® Ultra 594 maleimide	586	601	180000¹	0.91¹	0.07	0.05
iFluor® Ultra 647 maleimide	655	670	250000¹	0.39¹	0.07	0.07

References

View all 32 references: Citation Explorer

Targeting lung cancer with clinically relevant EGFR mutations using anti-EGFR RNA aptamer.
Authors: Thomas, Brian J and Guldenpfennig, Caitlyn and Guan, Yue and Winkler, Calvin and Beecher, Margaret and Beedy, Michaela and Berendzen, Ashley F and Ma, Lixin and Daniels, Mark A and Burke, Donald H and Porciani, David
Journal: Molecular therapy. Nucleic acids (2023): 102046

pH-responsive graphene oxide loaded with targeted peptide and anticancer drug for OSCC therapy.
Authors: Li, Ran and Gao, Ruifang and Zhao, Yingjiao and Zhang, Fang and Wang, Xiangyu and Li, Bing and Wang, Lu and Ma, Lixin and Du, Jie
Journal: Frontiers in oncology (2022): 930920

Near-Infrared Fluorescence Imaging of Carotid Plaques in an Atherosclerotic Murine Model.
Authors: Wu, Xiaotian and Daniel Ulumben, Amy and Long, Steven and Katagiri, Wataru and Wilks, Moses Q and Yuan, Hushan and Cortese, Brian and Yang, Chengeng and Kashiwagi, Satoshi and Choi, Hak Soo and Normandin, Marc D and El Fakhri, Georges and Zaman, Raiyan T
Journal: Biomolecules (2021)

Challenging a Preconception: Optoacoustic Spectrum Differs from the Optical Absorption Spectrum of Proteins and Dyes for Molecular Imaging.
Authors: Fuenzalida Werner, Juan Pablo and Huang, Yuanhui and Mishra, Kanuj and Janowski, Robert and Vetschera, Paul and Heichler, Christina and Chmyrov, Andriy and Neufert, Clemens and Niessing, Dierk and Ntziachristos, Vasilis and Stiel, Andre C
Journal: Analytical chemistry (2020)

CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model.
Authors: Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and de Jalón, Elvira García and Tenstad, Olav and Bjørge, Line and McCormack, Emmet
Journal: EBioMedicine (2020): 102783

Mechanistic profiling of the release kinetics of siRNA from lipidoid-polymer hybrid nanoparticles in vitro and in vivo after pulmonary administration.
Authors: Thanki, Kaushik and van Eetvelde, Delphine and Geyer, Antonia and Fraire, Juan and Hendrix, Remi and Van Eygen, Hannelore and Putteman, Emma and Sami, Haider and de Souza Carvalho-Wodarz, Cristiane and Franzyk, Henrik and Nielsen, Hanne Mørck and Braeckmans, Kevin and Lehr, Claus-Michael and Ogris, Manfred and Foged, Camilla
Journal: Journal of controlled release : official journal of the Controlled Release Society (2019): 82-93

Generation and characterization of novel recombinant anti-hERG1 scFv antibodies for cancer molecular imaging.
Authors: Duranti, Claudia and Carraresi, Laura and Sette, Angelica and Stefanini, Matteo and Lottini, Tiziano and Crescioli, Silvia and Crociani, Olivia and Iamele, Luisa and De Jonge, Hugo and Gherardi, Ermanno and Arcangeli, Annarosa
Journal: Oncotarget (2018): 34972-34989

Enhanced Release of Molecules upon Ultraviolet (UV) Light Irradiation from Photoresponsive Hydrogels Prepared from Bifunctional Azobenzene and Four-Arm Poly(ethylene glycol).
Authors: Rastogi, Shiva K and Anderson, Hailee E and Lamas, Joseph and Barret, Scott and Cantu, Travis and Zauscher, Stefan and Brittain, William J and Betancourt, Tania
Journal: ACS applied materials & interfaces (2018): 30071-30080

Phosphorothioate-Modified AP613-1 Specifically Targets GPC3 when Used for Hepatocellular Carcinoma Cell Imaging.
Authors: Dong, Lili and Zhou, Hongxin and Zhao, Menglong and Gao, Xinghui and Liu, Yang and Liu, Dongli and Guo, Wei and Hu, Hongwei and Xie, Qian and Fan, Jia and Lin, Jiang and Wu, Weizhong
Journal: Molecular therapy. Nucleic acids (2018): 376-386

In vivo fluorescence imaging of hepatocellular carcinoma using a novel GPC3-specific aptamer probe.
Authors: Zhao, Menglong and Dong, Lili and Liu, Zhuang and Yang, Shuohui and Wu, Weizhong and Lin, Jiang
Journal: Quantitative imaging in medicine and surgery (2018): 151-160