iFluor® Ultra 750 maleimide

The iFluor® Ultra series represents an enhancement of our established iFluor® dyes, optimized for antibody labeling in fluorescence imaging and flow cytometry. Within this series, iFluor® Ultra 750 is a bright NIR fluorescent dye excitable by 633 nm to 685 nm laser lines, with a peak emission at 773 nm. Antibody conjugates with iFluor® Ultra 750 demonstrate superior brightness compared to those labeled with spectrally similar dyes such as Cy7, Dylight 755, IRDye750, and Alexa Fluor® 750 under the same conditions. Additionally, iFluor® Ultra 750 maintains stable fluorescence across a pH range of 4 to 10. The maleimide derivative of iFluor® Ultra 750 is widely used for conjugation to thiol groups on proteins, oligonucleotide thiophosphates, or low molecular weight ligands. Conjugates with iFluor® Ultra 750 demonstrate higher fluorescence intensity and greater photostability compared to those with other spectrally similar fluorophores, enhancing their utility for advanced fluorescence applications. Fluorescent dye-conjugated antibodies are crucial for protein identification in various applications, including fluorescent cell imaging, flow cytometry, western blotting, and immunohistochemistry. The advantages of these conjugates include increased sensitivity, multiplexing capabilities, and ease of use, facilitating complex biological studies.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

Protocol

SDS

Catalog	Size	Price	Quantity
71683	1 mg	Price

References

View all 32 references: Citation Explorer

Targeting lung cancer with clinically relevant EGFR mutations using anti-EGFR RNA aptamer.

Authors:

Thomas, Brian J and Guldenpfennig, Caitlyn and Guan, Yue and Winkler, Calvin and Beecher, Margaret and Beedy, Michaela and Berendzen, Ashley F and Ma, Lixin and Daniels, Mark A and Burke, Donald H and Porciani, David

Journal:

Molecular therapy. Nucleic acids (2023): 102046

pH-responsive graphene oxide loaded with targeted peptide and anticancer drug for OSCC therapy.

Authors:

Li, Ran and Gao, Ruifang and Zhao, Yingjiao and Zhang, Fang and Wang, Xiangyu and Li, Bing and Wang, Lu and Ma, Lixin and Du, Jie

Journal:

Frontiers in oncology (2022): 930920

Near-Infrared Fluorescence Imaging of Carotid Plaques in an Atherosclerotic Murine Model.

Authors:

Wu, Xiaotian and Daniel Ulumben, Amy and Long, Steven and Katagiri, Wataru and Wilks, Moses Q and Yuan, Hushan and Cortese, Brian and Yang, Chengeng and Kashiwagi, Satoshi and Choi, Hak Soo and Normandin, Marc D and El Fakhri, Georges and Zaman, Raiyan T

Journal:

Biomolecules (2021)

Challenging a Preconception: Optoacoustic Spectrum Differs from the Optical Absorption Spectrum of Proteins and Dyes for Molecular Imaging.

Authors:

Fuenzalida Werner, Juan Pablo and Huang, Yuanhui and Mishra, Kanuj and Janowski, Robert and Vetschera, Paul and Heichler, Christina and Chmyrov, Andriy and Neufert, Clemens and Niessing, Dierk and Ntziachristos, Vasilis and Stiel, Andre C

Journal:

Analytical chemistry (2020)

CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model.

Authors:

Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and de Jalón, Elvira García and Tenstad, Olav and Bjørge, Line and McCormack, Emmet

Journal:

EBioMedicine (2020): 102783

Physical properties

Molecular weight	1208.27
Solvent	DMSO

Spectral properties

Absorbance (nm)	750
Correction factor (260 nm)	0.04
Correction factor (280 nm)	0.05
Correction factor (565 nm)	0.021
Correction factor (650 nm)	0.149
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	749
Emission (nm)	773
Quantum yield	0.32 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Labeling Antibodies & Biopolymers
Flyer: Antibody & Protein Labeling Dyes

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Fax
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Page updated on October 31, 2025