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iFluor® Ultra 647 maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 2672.16 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 654 |
| Correction Factor (260 nm) | 0.07 |
| Correction Factor (280 nm) | 0.07 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 655 |
| Emission (nm) | 670 |
| Quantum yield | 0.391 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| iFluor® Ultra 647 succinimidyl ester |
| Overview |  SDSProtocol |
Molecular weight 2672.16 | Absorbance (nm) 654 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.07 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 655 | Emission (nm) 670 | Quantum yield 0.391 |
The iFluor® Ultra series represents an enhancement of our established iFluor® dyes, optimized for antibody labeling in fluorescence imaging and flow cytometry. Within this series, iFluor® Ultra 647 is an exceptionally bright far-red fluorescent dye excitable by 594 nm or 633 nm laser lines, with a peak emission at 670 nm. Antibody conjugates with iFluor® Ultra 647 exhibit superior brightness compared to those labeled with Alexa Fluor® 647 under identical conditions. Additionally, iFluor® Ultra 647 maintains stable fluorescence across a pH range of 4 to 10. The maleimide derivative of iFluor® Ultra 647 is widely used for conjugation to thiol groups on proteins, oligonucleotide thiophosphates, or low molecular weight ligands. Conjugates with iFluor® Ultra 647 demonstrate higher fluorescence intensity and greater photostability compared to those with other spectrally similar fluorophores, enhancing their utility for advanced fluorescence applications. Fluorescent dye-conjugated antibodies are crucial for protein identification in various applications, including fluorescent cell imaging, flow cytometry, western blotting, and immunohistochemistry. The advantages of these conjugates include increased sensitivity, multiplexing capabilities, and ease of use, facilitating complex biological studies.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® Ultra 647 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 37.423 µL | 187.115 µL | 374.229 µL | 1.871 mL | 3.742 mL |
| 5 mM | 7.485 µL | 37.423 µL | 74.846 µL | 374.229 µL | 748.458 µL |
| 10 mM | 3.742 µL | 18.711 µL | 37.423 µL | 187.115 µL | 374.229 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 654 |
| Correction Factor (260 nm) | 0.07 |
| Correction Factor (280 nm) | 0.07 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 655 |
| Emission (nm) | 670 |
| Quantum yield | 0.391 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® Ultra 594 maleimide | 586 | 601 | 1800001 | 0.911 | 0.07 | 0.05 |
| iFluor® Ultra 750 maleimide | 749 | 773 | 2500001 | 0.321 | 0.04 | 0.05 |
References
View all 50 references: Citation Explorer
A Dual Strategy-In Vitro and In Silico-To Evaluate Human Antitetanus mAbs Addressing Their Potential Protective Action on TeNT Endocytosis in Primary Rat Neuronal Cells.
Authors: Lima, Cauã Pacheco and Barreiros, Gabriela Massaro and Oliveira, Adriele Silva Alves and de Souza, Marcelo Medina and Manieri, Tania Maria and Moro, Ana Maria
Journal: International journal of molecular sciences (2024)
Authors: Lima, Cauã Pacheco and Barreiros, Gabriela Massaro and Oliveira, Adriele Silva Alves and de Souza, Marcelo Medina and Manieri, Tania Maria and Moro, Ana Maria
Journal: International journal of molecular sciences (2024)
Examination of Pyroptosis by Flow Cytometry.
Authors: Chang, He and Chen, Zhi and Gao, Li and Cao, Hong and Wang, Yongqiang and Zheng, Shijun J
Journal: Journal of visualized experiments : JoVE (2024)
Authors: Chang, He and Chen, Zhi and Gao, Li and Cao, Hong and Wang, Yongqiang and Zheng, Shijun J
Journal: Journal of visualized experiments : JoVE (2024)
Enhanced fluorescence blinking of AF647 fluorophores in Mowiol via violet and UV light induced recovery for superior localization microscopy.
Authors: Bharadwaj, Anupam and Kumar, Amalesh and Mitra, Rumela and Jaganathan, Bithiah Grace and Boruah, Bosanta Ranjan
Journal: Methods and applications in fluorescence (2024)
Authors: Bharadwaj, Anupam and Kumar, Amalesh and Mitra, Rumela and Jaganathan, Bithiah Grace and Boruah, Bosanta Ranjan
Journal: Methods and applications in fluorescence (2024)
Three species multiplexing of fluorescent dyes and gold nanoclusters recovered with fluorescence lifetime correlation spectroscopy.
Authors: Kayyil Veedu, Malavika and Hajda, Agata and Olesiak-Bańska, Joanna and Wenger, Jérôme
Journal: Biochimica et biophysica acta. General subjects (2024): 130611
Authors: Kayyil Veedu, Malavika and Hajda, Agata and Olesiak-Bańska, Joanna and Wenger, Jérôme
Journal: Biochimica et biophysica acta. General subjects (2024): 130611
Vanadocene-functionalized mesoporous silica nanoparticles: platforms for the development of theranostic materials against breast cancer.
Authors: Iorhemba, Michael Aondona and Álvarez-Conde, Javier and Díaz-García, Diana and Méndez-Arriaga, José Manuel and García-Almodóvar, Victoria and Ovejero-Paredes, Karina and Idris, Sulaiman Ola and Shallangwa, Gideon Adamu and Abdulkadir, Ibrahim and Prashar, Sanjiv and Filice, Marco and Gómez-Ruiz, Santiago
Journal: Biomedical materials (Bristol, England) (2024)
Authors: Iorhemba, Michael Aondona and Álvarez-Conde, Javier and Díaz-García, Diana and Méndez-Arriaga, José Manuel and García-Almodóvar, Victoria and Ovejero-Paredes, Karina and Idris, Sulaiman Ola and Shallangwa, Gideon Adamu and Abdulkadir, Ibrahim and Prashar, Sanjiv and Filice, Marco and Gómez-Ruiz, Santiago
Journal: Biomedical materials (Bristol, England) (2024)
Amphiphilic Poly(2-oxazoline)s with Glycine-Containing Hydrophobic Blocks Tailored for Panobinostat- and Imatinib-Loaded Micelles.
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
Rapid and portable bunyavirus SFTSV RNA testing utilizing catalytic hairpin assembly coupled with lateral flow immunoassay.
Authors: Chen, Lin and Ma, Mengyin and Zou, Mingyuan and Zhao, Liwei and Ou, Mingrong and Geng, Yu and Li, Chuang and Shen, Han and Chen, Yuxin
Journal: Microbiology spectrum (2023): e0214423
Authors: Chen, Lin and Ma, Mengyin and Zou, Mingyuan and Zhao, Liwei and Ou, Mingrong and Geng, Yu and Li, Chuang and Shen, Han and Chen, Yuxin
Journal: Microbiology spectrum (2023): e0214423
USE OF RECOMBINANT S1 PROTEIN WITH hFc FOR ANALYSIS OF SARS-COV-2 ADSORPTION AND EVALUATION OF DRUGS THAT INHIBIT ENTRY INTO VERO E6 CELLS.
Authors: Couto, Jéssica Carla Martins and Vidal, Taís and Decker, Eduardo Reichert and Santurio, Janio M and de Mello, Carlos Fernando and Pillat, Micheli Mainardi
Journal: Immunology letters (2023)
Authors: Couto, Jéssica Carla Martins and Vidal, Taís and Decker, Eduardo Reichert and Santurio, Janio M and de Mello, Carlos Fernando and Pillat, Micheli Mainardi
Journal: Immunology letters (2023)
Click-Functionalization of Silanized Carbon Nanotubes: From Inorganic Heterostructures to Biosensing Nanohybrids.
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Manoharan, Gririraj and Bösel, Petra and Thien, Jannis and Holtmannspötter, Michael and Meingast, Laura and Schmidt, Mercedes and Eickmeier, Henning and Haase, Markus and Maultzsch, Janina and Steinhart, Martin and Wollschläger, Joachim and Palma, Matteo and Meyer, Carola
Journal: Molecules (Basel, Switzerland) (2023)
Possible frequent multiple mitochondrial DNA copies in a single nucleoid in HeLa cells.
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788
Authors: Pavluch, Vojtěch and Špaček, Tomáš and Engstová, Hana and Dlasková, Andrea and Ježek, Petr
Journal: Scientific reports (2023): 5788