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iFluor® Ultra 594 maleimide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1097.12 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 585 |
| Correction Factor (260 nm) | 0.07 |
| Correction Factor (280 nm) | 0.05 |
| Extinction coefficient (cm -1 M -1) | 1800001 |
| Excitation (nm) | 586 |
| Emission (nm) | 601 |
| Quantum yield | 0.911 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| iFluor® Ultra 594 succinimidyl ester |
| Overview |  SDSProtocol |
Molecular weight 1097.12 | Absorbance (nm) 585 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.05 | Extinction coefficient (cm -1 M -1) 1800001 | Excitation (nm) 586 | Emission (nm) 601 | Quantum yield 0.911 |
The iFluor® Ultra series represents an enhancement of our established iFluor® dyes, optimized for antibody labeling in fluorescence imaging and flow cytometry. Within this series, iFluor® Ultra 594 is an exceptionally bright red fluorescent dye excitable by 561 nm or 594 nm laser lines, with a peak emission at 601 nm. Antibody conjugates with iFluor® Ultra 594 exhibit superior brightness compared to those labeled with Alexa Fluor® 594 under identical conditions. Additionally, iFluor® Ultra 594 maintains stable fluorescence across a pH range of 4 to 10. The maleimide derivative of iFluor® Ultra 594 is widely used for conjugation to thiol groups on proteins, oligonucleotide thiophosphates, or low molecular weight ligands. Conjugates with iFluor® Ultra 594 demonstrate higher fluorescence intensity and greater photostability compared to those with other spectrally similar fluorophores, enhancing their utility for advanced fluorescence applications. Fluorescent dye-conjugated antibodies are crucial for protein identification in various applications, including fluorescent cell imaging, flow cytometry, western blotting, and immunohistochemistry. The advantages of these conjugates include increased sensitivity, multiplexing capabilities, and ease of use, facilitating complex biological studies.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® Ultra 594 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 91.148 µL | 455.739 µL | 911.477 µL | 4.557 mL | 9.115 mL |
| 5 mM | 18.23 µL | 91.148 µL | 182.295 µL | 911.477 µL | 1.823 mL |
| 10 mM | 9.115 µL | 45.574 µL | 91.148 µL | 455.739 µL | 911.477 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 585 |
| Correction Factor (260 nm) | 0.07 |
| Correction Factor (280 nm) | 0.05 |
| Extinction coefficient (cm -1 M -1) | 1800001 |
| Excitation (nm) | 586 |
| Emission (nm) | 601 |
| Quantum yield | 0.911 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® Ultra 647 maleimide | 655 | 670 | 2500001 | 0.391 | 0.07 | 0.07 |
| iFluor® Ultra 750 maleimide | 749 | 773 | 2500001 | 0.321 | 0.04 | 0.05 |
References
View all 50 references: Citation Explorer
Correlative super-resolution microscopy with deep UV reactivation.
Authors: Prakash, Kirti
Journal: Journal of microscopy (2023)
Authors: Prakash, Kirti
Journal: Journal of microscopy (2023)
Amphiphilic Poly(2-oxazoline)s with Glycine-Containing Hydrophobic Blocks Tailored for Panobinostat- and Imatinib-Loaded Micelles.
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
Authors: Göppert, Natalie E and Quader, Sabina and Van Guyse, Joachim F R and Weber, Christine and Kataoka, Kazunori and Schubert, Ulrich S
Journal: Biomacromolecules (2023): 5915-5925
Anatomical analyses of collateral prefrontal cortex projections to the basolateral amygdala and the nucleus accumbens core in rats.
Authors: Shih, Cheng-Wei and Chang, Chun-Hui
Journal: Brain structure & function (2023)
Authors: Shih, Cheng-Wei and Chang, Chun-Hui
Journal: Brain structure & function (2023)
A model eye for fluorescent characterization of retinal cultures and tissues.
Authors: Ferraro, G and Gigante, Y and Pitea, M and Mautone, L and Ruocco, G and Di Angelantonio, S and Leonetti, M
Journal: Scientific reports (2023): 10983
Authors: Ferraro, G and Gigante, Y and Pitea, M and Mautone, L and Ruocco, G and Di Angelantonio, S and Leonetti, M
Journal: Scientific reports (2023): 10983
Total Flavonoids of Polygala fallax Hemsl Induce Apoptosis of Human Ectopic Endometrial Stromal Cells through PI3K/AKT/Bcl-2 Signaling Pathway.
Authors: Zhong, Chuanmei and Ju, Gongchenhao and Yang, Sufang and Zhao, Xiangpei and Chen, Jixiang and Li, Ning
Journal: Gynecologic and obstetric investigation (2023): 197-213
Authors: Zhong, Chuanmei and Ju, Gongchenhao and Yang, Sufang and Zhao, Xiangpei and Chen, Jixiang and Li, Ning
Journal: Gynecologic and obstetric investigation (2023): 197-213
Resolving arteriolar wall structures in mouse brain in vivo with three-photon microscopy.
Authors: Qin, Mengyuan and Huang, Jie and Zhong, Jincheng and Zhang, Yingxian and Tong, Shen and Cheng, Hui and Deng, Xiangquan and Zheng, Lei and Zhang, Wanjian and Qiu, Ping and Wang, Ke
Journal: Journal of biophotonics (2023): e202200365
Authors: Qin, Mengyuan and Huang, Jie and Zhong, Jincheng and Zhang, Yingxian and Tong, Shen and Cheng, Hui and Deng, Xiangquan and Zheng, Lei and Zhang, Wanjian and Qiu, Ping and Wang, Ke
Journal: Journal of biophotonics (2023): e202200365
Development and Application of a Cell-Based Assay for LRP4 Antibody Associated With Myasthenia Gravis.
Authors: Chung, Hye Yoon and Kim, Min Ju and Kim, Seung Woo and Oh, Jeeyoung and Shin, Ha Young
Journal: Journal of clinical neurology (Seoul, Korea) (2023): 60-66
Authors: Chung, Hye Yoon and Kim, Min Ju and Kim, Seung Woo and Oh, Jeeyoung and Shin, Ha Young
Journal: Journal of clinical neurology (Seoul, Korea) (2023): 60-66
A modified dentine infection model with fluorescent lipopolysaccharide and lipopolysaccharides sampling technique to compare XP-Endo finisher and passive ultrasonic irrigation.
Authors: Alfirdous, Rayyan A and Alquiria, Theeb A and Jacinto, Rogerio C and Martinho, Frederico C
Journal: International endodontic journal (2022): 1081-1090
Authors: Alfirdous, Rayyan A and Alquiria, Theeb A and Jacinto, Rogerio C and Martinho, Frederico C
Journal: International endodontic journal (2022): 1081-1090
Comparison of GentleWave system and passive ultrasonic irrigation with minimally invasive and conventional instrumentation against LPS in infected root canals.
Authors: Velardi, Johnathan P and Alquria, Theeb A and Alfirdous, Rayyan A and Corazza, Bruna J M and Gomes, Ana P M and Silva, Eduardo G and Griffin, Ina L and Tordik, Patricia A and Martinho, Frederico C
Journal: Scientific reports (2022): 4894
Authors: Velardi, Johnathan P and Alquria, Theeb A and Alfirdous, Rayyan A and Corazza, Bruna J M and Gomes, Ana P M and Silva, Eduardo G and Griffin, Ina L and Tordik, Patricia A and Martinho, Frederico C
Journal: Scientific reports (2022): 4894
The procoagulant effects of extracellular vesicles derived from hypoxic endothelial cells can be selectively inhibited by inorganic nitrite.
Authors: Whelan, Cass and Burnley-Hall, Nicholas and Morris, Keith and Rees, D Aled and James, Philip E
Journal: Nitric oxide : biology and chemistry (2022): 6-18
Authors: Whelan, Cass and Burnley-Hall, Nicholas and Morris, Keith and Rees, D Aled and James, Philip E
Journal: Nitric oxide : biology and chemistry (2022): 6-18