iFluor® 790 acid
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, including widely used small indocarbocyanine dyes. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 790 is designed to label proteins and other biomolecules with near-infrared fluorescence. Conjugates prepared with iFluor® 790 have excitation and emission spectra similar to that of indocyanine green (ICG) and the IRDye® 800 dye, with 783/814 nm excitation/emission maxima. iFluor® 790 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in-vivo imaging applications or other imaging applications requiring NIR detections, such as the two-color western applications with the LI-COR® Odyssey® infrared imaging system.
Example protocol
AT A GLANCE
Important notes
It is important to store at <-15 °C and should be stored in cool, dark place.
It can be used within 24 months from the date of receipt.
PREPARATION OF WORKING SOLUTION
iFluor™ 790 acid working solution:
Add DMF, DMSO or water to make iFluor™ 790 acid working solution of desired concentration.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 790 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 78.23 µL | 391.151 µL | 782.301 µL | 3.912 mL | 7.823 mL |
5 mM | 15.646 µL | 78.23 µL | 156.46 µL | 782.301 µL | 1.565 mL |
10 mM | 7.823 µL | 39.115 µL | 78.23 µL | 391.151 µL | 782.301 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 800 acid | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
iFluor® 810 acid | 811 | 822 | 2500001 | 0.051 | 0.09 | 0.15 |
iFluor® 820 acid | 822 | 850 | 2500001 | 0.11 | 0.16 | |
iFluor® 860 acid | 853 | 878 | 2500001 | 0.1 | 0.14 | |
iFluor® 790 Styramide *Superior Replacement for Alexa Fluor 790 tyramide* | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 840 acid | 836 | 879 | 2000001 | - | 0.2 | 0.09 |
iFluor® 830 acid | 830 | 867 | - | - | - | - |
iFluor® 670 acid | 671 | 682 | 2000001 | 0.551 | 0.03 | 0.033 |
iFluor® 350 acid | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
Show More (4) |
Citations
View all 5 citations: Citation Explorer
Lipid nanoparticulate drug delivery system for the treatment of hepatic fibrosis
Authors: Mukherjee, Swarupananda and Dutta, Ayon and Ash, Dipanjana
Journal: Archives of Hepatitis Research (2021): 001--003
Authors: Mukherjee, Swarupananda and Dutta, Ayon and Ash, Dipanjana
Journal: Archives of Hepatitis Research (2021): 001--003
Lipid-based nanoparticle technologies for liver targeting
Authors: B{\"o}ttger, Roland and Pauli, Griffin and Chao, Po-Han and Fayez, Nojoud AL and Hohenwarter, Lukas and Li, Shyh-Dar
Journal: Advanced drug delivery reviews (2020): 79--101
Authors: B{\"o}ttger, Roland and Pauli, Griffin and Chao, Po-Han and Fayez, Nojoud AL and Hohenwarter, Lukas and Li, Shyh-Dar
Journal: Advanced drug delivery reviews (2020): 79--101
Self-assembly and directed assembly of lipid nanocarriers for prevention of liver fibrosis in obese rats: a comparison with the therapy of bariatric surgery
Authors: Chen, Chun-Han and Chen, Chih-Jung and Elzoghby, Ahmed O and Yeh, Ta-Sen and Fang, Jia-You
Journal: Nanomedicine (2018)
Authors: Chen, Chun-Han and Chen, Chih-Jung and Elzoghby, Ahmed O and Yeh, Ta-Sen and Fang, Jia-You
Journal: Nanomedicine (2018)
Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)
Oleic acid-based nanosystems for mitigating acute respiratory distress syndrome in mice through neutrophil suppression: how the particulate size affects therapeutic efficiency
Authors: Yu, Huang-Ping and Liu, Fu-Chao and Umoro, Ani and Lin, Zih-Chan
Authors: Yu, Huang-Ping and Liu, Fu-Chao and Umoro, Ani and Lin, Zih-Chan
References
View all 18 references: Citation Explorer
In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
Authors: Szabo A, Vollmar B, Boros M, Menger MD.
Journal: J Surg Res. (2007)
Authors: Szabo A, Vollmar B, Boros M, Menger MD.
Journal: J Surg Res. (2007)
A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Authors: Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: Cancer Res (2007): 2791
Fluorescence imaging in vivo: recent advances
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
Authors: Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: Photochem Photobiol (2007): 1157
In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
Authors: Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: J Immunol Methods (2007): 65
Authors: Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: J Immunol Methods (2007): 65
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