iFluor® 830 acid
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1359.40 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
iFluor® 830 succinimidyl ester |
iFluor® 830 maleimide |
Overview | ![]() ![]() |
See also: iFluor® Dyes and Kits
Molecular weight 1359.40 | Absorbance (nm) 830 | Excitation (nm) 830 | Emission (nm) 867 |
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emissions at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 830 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 73.562 µL | 367.809 µL | 735.619 µL | 3.678 mL | 7.356 mL |
5 mM | 14.712 µL | 73.562 µL | 147.124 µL | 735.619 µL | 1.471 mL |
10 mM | 7.356 µL | 36.781 µL | 73.562 µL | 367.809 µL | 735.619 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fifluor_830.png&w=2048&q=50)
![spectrum](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fspectra%2Fifluor_830.png&w=2048&q=50)
Spectral properties
Absorbance (nm) | 830 |
Excitation (nm) | 830 |
Emission (nm) | 867 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 790 acid | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 acid | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
iFluor® 810 acid | 811 | 822 | 2500001 | 0.051 | 0.09 | 0.15 |
iFluor® 820 acid | 822 | 850 | 2500001 | 0.11 | 0.16 | |
iFluor® 860 acid | 853 | 878 | 2500001 | 0.1 | 0.14 | |
iFluor® 840 acid | 836 | 879 | 2000001 | - | 0.2 | 0.09 |
Images
![iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have the excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis.](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-830-acid%2Ffigure-for-ifluor-830-acid_wWueD.png&w=3840&q=75)
Figure 1. iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have the excitation and emission in the IR range. iFluor® 830 dye emission is a unique color for spectrum flow cytometry as it is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis.
References
View all 50 references: Citation Explorer
Viscosity Effects on Excited-State Dynamics of Indocyanine Green for Phototheranostic.
Authors: Liao, Keyue and Pan, Yonghui and Wu, Zizi and Yao, Weiyun and Miao, Xiaofei and Lu, Xiaomei and Fan, Quli and Hu, Wenbo
Journal: Chemistry, an Asian journal (2022): e202200112
Authors: Liao, Keyue and Pan, Yonghui and Wu, Zizi and Yao, Weiyun and Miao, Xiaofei and Lu, Xiaomei and Fan, Quli and Hu, Wenbo
Journal: Chemistry, an Asian journal (2022): e202200112
Multifunctional Nanoparticles Co-Loaded with Perfluoropropane, Indocyanine Green, and Methotrexate for Enhanced Multimodal Imaging of Collagen-Induced Arthritis.
Authors: He, Xinyu and Chen, Huanhuan and Chang, Shufang and Zhu, Shenyin
Journal: Molecular pharmaceutics (2022)
Authors: He, Xinyu and Chen, Huanhuan and Chang, Shufang and Zhu, Shenyin
Journal: Molecular pharmaceutics (2022)
Indocyanine green guidance for combined robotic pelvic and para-aortic sentinel node mapping in cervical cancer.
Authors: Van Trappen, Philippe
Journal: Surgical oncology (2022): 101745
Authors: Van Trappen, Philippe
Journal: Surgical oncology (2022): 101745
Leak after sleeve gastrectomy with positive intraoperative indocyanine green test: Avoidable scenario?
Authors: Pavone, Giovanna and Tartaglia, Nicola and Pacilli, Mario and Maddalena, Francesca and Petruzzelli, Fabio and Ambrosi, Antonio
Journal: International journal of surgery case reports (2022): 107168
Authors: Pavone, Giovanna and Tartaglia, Nicola and Pacilli, Mario and Maddalena, Francesca and Petruzzelli, Fabio and Ambrosi, Antonio
Journal: International journal of surgery case reports (2022): 107168
Determination of surgical margins in laparoscopic parenchyma-sparing hepatectomy of neuroendocrine tumors liver metastases using indocyanine green fluorescence imaging.
Authors: Wang, Gaoming and Luo, Ying and Qi, Weijun and Yuan, Chunhui and Xiu, Dianrong
Journal: Surgical endoscopy (2022): 4408-4416
Authors: Wang, Gaoming and Luo, Ying and Qi, Weijun and Yuan, Chunhui and Xiu, Dianrong
Journal: Surgical endoscopy (2022): 4408-4416
Indocyanine green colonic perfusion demonstration following robotic da Vinci X inferior mesenteric artery ligation for the treatment of type II endoleak.
Authors: Lambert, Joel and Al Majid, Sulaymaan and Salaman, Robert and Gavan, Duncan and Sheikh, Adnan and Gill, Michael
Journal: The international journal of medical robotics + computer assisted surgery : MRCAS (2022): e2407
Authors: Lambert, Joel and Al Majid, Sulaymaan and Salaman, Robert and Gavan, Duncan and Sheikh, Adnan and Gill, Michael
Journal: The international journal of medical robotics + computer assisted surgery : MRCAS (2022): e2407
A New Method to Identify Air Leaks After Pulmonary Resection Using Indocyanine Green Aerosol.
Authors: Yokota, Naoya and Go, Tetsuhiko and Otsuki, Yasuhiro and Chang, Sung Soo and Misaki, Noriyuki and Yokomise, Hiroyasu
Journal: The Annals of thoracic surgery (2022)
Authors: Yokota, Naoya and Go, Tetsuhiko and Otsuki, Yasuhiro and Chang, Sung Soo and Misaki, Noriyuki and Yokomise, Hiroyasu
Journal: The Annals of thoracic surgery (2022)
Laparoscopic Indocyanine Green Fluorescence Imaging for Intrahepatic Cholangiocarcinoma.
Authors: Piccolo, Gaetano and Barabino, Matteo and Lecchi, Francesca and Santambrogio, Roberto and Nava, Claudia and Opocher, Enrico and Bianchi, Paolo Pietro
Journal: The American surgeon (2022): 31348221103659
Authors: Piccolo, Gaetano and Barabino, Matteo and Lecchi, Francesca and Santambrogio, Roberto and Nava, Claudia and Opocher, Enrico and Bianchi, Paolo Pietro
Journal: The American surgeon (2022): 31348221103659
Indocyanine green fluorescence: A surgeon's tool for the surgical approach of gallstone ileus.
Authors: Nieto, Luis Alejandro and Cabrera-Vargas, Luis Felipe and Lozada-Martínez, Ivan David and Guardo-Carmona, Daniela and Contreras, Martin and Pedraza, Mauricio and Narvaez-Rojas, Alexis Rafael
Journal: Clinical case reports (2022): e05873
Authors: Nieto, Luis Alejandro and Cabrera-Vargas, Luis Felipe and Lozada-Martínez, Ivan David and Guardo-Carmona, Daniela and Contreras, Martin and Pedraza, Mauricio and Narvaez-Rojas, Alexis Rafael
Journal: Clinical case reports (2022): e05873
Effect of introduction of indocyanine green angiography of parathyroid glands on postoperative hypoparathyroidism after total thyroidectomy.
Authors: Demarchi, Marco Stefano and Flaris, Alexandros N and Fortuny, Jordi Vidal and Bedat, Benoit and Karenovics, Wolfram and Triponez, Frederic
Journal: BJS open (2022)
Authors: Demarchi, Marco Stefano and Flaris, Alexandros N and Fortuny, Jordi Vidal and Bedat, Benoit and Karenovics, Wolfram and Triponez, Frederic
Journal: BJS open (2022)