iFluor® 510 maleimide

iFluor® 510 maleimide selectively reacts with thiol group of a biomolecule. It is widely used to label the reduced antibodies. iFluor®510 is a new fluorescent dye belonging to the iFluor® family of dyes. These dyes are known for their bright fluorescence, photostability, and compatibility with various imaging techniques and instruments. iFluor 510 emits green fluorescence when excited with light in the blue to green range (around 488 to 514 nm). Its emission peak is typically around 520 to 530 nm. The number "510" in its name represents the approximate maximum excitation wavelength. Like other iFluor dyes, AAT Bioquest offers a variety of iFluor 510 derivatives that can be readily used to develop conjugates for biological research and imaging applications. They can be conjugated to various biomolecules, such as antibodies, proteins, nucleic acids, and small molecules, for fluorescence microscopy, flow cytometry, immunohistochemistry, and other fluorescence-based assays.

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 546 maleimide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
iFluor® 720 maleimide	716	740	240000¹	0.14¹	0.15	0.13
iFluor® 560 maleimide	560	571	120000¹	0.57¹	0.0482	0.069
Show More (22)

References

View all 50 references: Citation Explorer

Recent Advances in N-Heterocyclic Small Molecules for Synthesis and Application in Direct Fluorescence Cell Imaging.
Authors: Li, Yanan and Liu, Tao and Sun, Jianan
Journal: Molecules (Basel, Switzerland) (2023)

Activatable Nanoprobe with Aggregation-Induced Dual Fluorescence and Photoacoustic Signal Enhancement for Tumor Precision Imaging and Radiotherapy.
Authors: Yuan, Meng and Fang, Xiao and Wu, Ying and Xu, Yuanji and Feng, Hongjuan and Mu, Jing and Chen, Zhongxiang and Lin, Yuhong and Fu, Qinrui and Du, Wei and Yang, Huanghao and Song, Jibin
Journal: Analytical chemistry (2022): 5204-5211

Labeling of Proteins for Single-Molecule Fluorescence Spectroscopy.
Authors: Zosel, Franziska and Holla, Andrea and Schuler, Benjamin
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 207-233

Design and Validation of Site-Specifically Labeled Single-Domain Antibody-Based Tracers for in Vivo Fluorescence Imaging and Image-Guided Surgery.
Authors: Declerck, Noemi B and Mateusiak, Lukasz and Hernot, Sophie
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 395-407

Sensitive and specific detection of breast cancer lymph node metastasis through dual-modality magnetic particle imaging and fluorescence molecular imaging: a preclinical evaluation.
Authors: Wang, Guorong and Li, Wenzhe and Shi, Guangyuan and Tian, Yu and Kong, Lingyan and Ding, Ning and Lei, Jing and Jin, Zhengyu and Tian, Jie and Du, Yang
Journal: European journal of nuclear medicine and molecular imaging (2022): 2723-2734

Page updated on November 21, 2024

Ordering information

Price
Unit size
Catalog Number	1425
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international