iFluor® 540 maleimide

iFluor® 540 maleimide selectively reacts with thiol group of a biomolecule. It is widely used to label the reduced antibodies and other thiol-containing biomolecules such as peptides and thiol-modified oligos. iFluor®540 is a fluorophore from the iFluor family, which is known for its bright red fluorescence and compatibility with various fluorescence techniques and instruments. When excited with light in the green to yellow range (around 530 to 545 nm), iFluor 540 emits red fluorescence with an emission peak typically around 560 to 570 nanometers. iFluor 540 can be conjugated to a variety of biomolecules, including antibodies, proteins, nucleic acids, and small molecules, enabling their visualization and tracking in cells and tissues. It is commonly used in fluorescence microscopy, immunohistochemistry, flow cytometry, and other fluorescence-based assays.

Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 maleimide	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 488 maleimide	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 555 maleimide	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 647 maleimide	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 maleimide	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 maleimide	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 maleimide	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 maleimide	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 800 maleimide	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 maleimide	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 820 maleimide	822	850	250000¹		0.11	0.16
iFluor® 860 maleimide	853	878	250000¹		0.1	0.14
iFluor® 532 maleimide	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 594 maleimide	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 405 maleimide	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 430 maleimide	433	498	40000¹	0.78¹	0.68	0.3
iFluor® 568 maleimide	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 633 maleimide	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 450 maleimide	451	502	40000¹	0.82¹	0.45	0.27
iFluor® 460 maleimide	468	493	80000¹	~0.8¹	0.98	0.46
iFluor® 665 maleimide	667	692	110,000¹	0.22¹	0.12	0.09
iFluor® 546 maleimide	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 840 maleimide	836	879	200000¹	-	0.2	0.09
iFluor® 770 maleimide	777	797	250000¹	0.16	0.09	0.08
iFluor® 780 maleimide	784	808	250000¹	0.16¹	0.13	0.12
iFluor® 830 maleimide	830	867	-	-	-	-
iFluor® 514 maleimide	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 660 maleimide	663	678	250000¹	0.26¹	0.07	0.08
iFluor® 670 maleimide	671	682	200000¹	0.55¹	0.03	0.033
iFluor® 720 maleimide	716	740	240000¹	0.14¹	0.15	0.13
iFluor® 560 maleimide	560	571	120000¹	0.57¹	0.0482	0.069
Show More (22)

References

View all 8 references: Citation Explorer

A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility.
Authors: Shrivastava, Abhishek and Berg, Howard C
Journal: Science advances (2020): eaay6616

Protein Environment and DNA Orientation Affect Protein-Induced Cy3 Fluorescence Enhancement.
Authors: Nguyen, Binh and Ciuba, Monika A and Kozlov, Alexander G and Levitus, Marcia and Lohman, Timothy M
Journal: Biophysical journal (2019): 66-73

Electrical readout of protein microarrays on regular glass slides.
Authors: Bonilla, Diana and Mallén, Maria and de la Rica, Roberto and Fernández-Sánchez, César and Baldi, Antonio
Journal: Analytical chemistry (2011): 1726-31

Toward a solid-phase nucleic acid hybridization assay within microfluidic channels using immobilized quantum dots as donors in fluorescence resonance energy transfer.
Authors: Chen, Lu and Algar, W Russ and Tavares, Anthony J and Krull, Ulrich J
Journal: Analytical and bioanalytical chemistry (2011): 133-41

Thiazole orange and Cy3: improvement of fluorescent DNA probes with use of short range electron transfer.
Authors: Menacher, Florian and Rubner, Moritz and Berndl, Sina and Wagenknecht, Hans-Achim
Journal: The Journal of organic chemistry (2008): 4263-6

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