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iFluor® 540 succinimidyl ester

iFluor® 540 succinimidyl ester is the most convenient reactive form of iFluor® 540 dyes that can be readily used for labeling proteins such as antibodies. iFluor® 540 is a fluorophore from the iFluor® family, which is known for its bright red fluorescence and compatibility with various fluorescence techniques and instruments. When excited with light in the green to yellow range (around 530 to 545 nm), iFluor® 540 emits red fluorescence with an emission peak typically around 560 to 570 nanometers. iFluor® 540 can be conjugated to a variety of biomolecules, including antibodies, proteins, nucleic acids, and small molecules, enabling their visualization and tracking in cells and tissues. It is commonly used in fluorescence microscopy, immunohistochemistry, flow cytometry, and other fluorescence-based assays. The iFluor® dyes, including iFluor® 540, are designed to exhibit high brightness, photostability, and minimal background noise, making them ideal for demanding imaging applications. They are also compatible with multiplexing experiments, allowing for simultaneous visualization of multiple targets using different iFluor® dyes with distinct emission wavelengths.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 succinimidyl ester3454502000010.9510.830.23
iFluor® 405 succinimidyl ester4034273700010.9110.480.77
iFluor® 488 succinimidyl ester4915167500010.910.210.11
iFluor® 514 succinimidyl ester5115277500010.8310.2650.116
iFluor® 532 succinimidyl ester5375609000010.6810.260.16
iFluor® 555 succinimidyl ester55757010000010.6410.230.14
iFluor® 594 succinimidyl ester58760320000010.5310.050.04
iFluor® 633 succinimidyl ester64065425000010.2910.0620.044
iFluor® 647 succinimidyl ester65667025000010.2510.030.03
iFluor® 660 succinimidyl ester66367825000010.2610.070.08
iFluor® 680 succinimidyl ester68470122000010.2310.0970.094
iFluor® 700 succinimidyl ester69071322000010.2310.090.04
iFluor® 750 succinimidyl ester75777927500010.1210.0440.039
iFluor® 610 succinimidyl ester61062811000010.8510.320.49
iFluor® 710 succinimidyl ester71673915000010.6010.120.07
iFluor® 790 succinimidyl ester78781225000010.1310.10.09
iFluor® 800 succinimidyl ester80182025000010.1110.030.08
iFluor® 810 succinimidyl ester81182225000010.0510.090.15
iFluor® 820 succinimidyl ester82285025000010.110.16
iFluor® 860 succinimidyl ester85387825000010.10.14
iFluor® 546 succinimidyl ester54155710000010.6710.250.15
iFluor® 568 succinimidyl ester56858710000010.5710.340.15
iFluor® 430 succinimidyl ester4334984000010.7810.680.3
iFluor® 450 succinimidyl ester4515024000010.8210.450.27
iFluor® 840 succinimidyl ester8368792000001-0.20.09
iFluor® 560 succinimidyl ester56057112000010.5710.04820.069
iFluor® 670 succinimidyl ester67168220000010.5510.030.033
iFluor® 460 succinimidyl ester468493800001~0.810.980.46
iFluor® 440 succinimidyl ester4344804000010.6710.3520.229
iFluor® 665 succinimidyl ester667692110,00010.2210.120.09
iFluor® 690 succinimidyl ester68570422000010.3010.090.06
iFluor® 720 succinimidyl ester71674024000010.1410.150.13
iFluor® 740 succinimidyl ester74076422500010.2010.160.16
iFluor® 597 succinimidyl ester59861810000010.710.3350.514
iFluor® 770 succinimidyl ester77779725000010.160.090.08
iFluor® 780 succinimidyl ester78480825000010.1610.130.12
iFluor® 570 succinimidyl ester55757012000010.581--
iFluor® 830 succinimidyl ester830867----
iFluor® 675 succinimidyl ester683700---0.066
iFluor® 620 succinimidyl ester621636---0.04
iFluor® 605 succinimidyl ester603623----
iFluor® 625 succinimidyl ester624640----
iFluor® 510 succinimidyl ester511530----
iFluor® 445 succinimidyl ester446558----
iFluor® 500 succinimidyl ester501520----
Show More (36)

References

View all 39 references: Citation Explorer
Gradient-dependent release of the model drug TRITC-dextran from FITC-labeled BSA hydrogel nanocarriers in the hair follicles of porcine ear skin.
Authors: Tran, Ngo Bich Nga Nathalie and Knorr, Fanny and Mak, Wing Cheung and Cheung, Kwan Yee and Richter, Heike and Meinke, Martina and Lademann, Jürgen and Patzelt, Alexa
Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenst (2017): 12-16
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells.
Authors: Wunsch, Christopher M and Lewis, Janina P
Journal: Journal of visualized experiments : JoVE (2015): e53408
Cell-matrix and cell-cell interactions of human gingival fibroblasts on three-dimensional nanofibrous gelatin scaffolds.
Authors: Sachar, Ashneet and Strom, T Amanda and San Miguel, Symone and Serrano, Maria J and Svoboda, Kathy K H and Liu, Xiaohua
Journal: Journal of tissue engineering and regenerative medicine (2014): 862-73
Sexually dimorphic and developmentally regulated expression of tubulin-specific chaperone protein A in the LMAN of zebra finches.
Authors: Qi, L M and Wade, J
Journal: Neuroscience (2013): 182-90
Mefloquine damage vestibular hair cells in organotypic cultures.
Authors: Yu, Dongzhen and Ding, Dalian and Jiang, Haiyan and Stolzberg, Daniel and Salvi, Richard
Journal: Neurotoxicity research (2011): 51-8
Page updated on November 21, 2024

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Catalog Number1426
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Physical properties

Molecular weight

853.96

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.105

Excitation (nm)

540

Emission (nm)

557

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure