iFluor® 605 maleimide

iFluor® 605 maleimide is a red-fluorescent, thiol-reactive dye designed for covalent labeling of reduced antibodies, peptides, and other sulfhydryl-containing biomolecules.

Thiol-Reactive Labeling: Selectively reacts with sulfhydryl groups to form stable conjugates.
Spectral Properties: Excitation/emission maxima of 603/623 nm.
Bright Fluorescence: Produces strong red fluorescence upon excitation in the 580–630 nm range.
Applications: Suitable for fluorescence microscopy, immunohistochemistry, and flow cytometry.

Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.

Protocol

SDS

Catalog	Size	Price	Quantity
1421	1 mg	Price

Overview

iFluor® 605 maleimide selectively reacts with thiol group of a biomolecule. It is widely used to label reduced antibodies and other thiol-containing biomolecules such as peptides and thiol-modified oligos. iFluor®605 is a fluorophore from the iFluor® family, which is known for its bright red fluorescence and compatibility with various fluorescence techniques and instruments. When excited with light in the red range (around 580 to 630 nm), iFluor® 605 emits strong red fluorescence. iFluor® 605 can be conjugated to a variety of biomolecules, including antibodies, proteins, nucleic acids, and small molecules, enabling their visualization and tracking in cells and tissues. It is commonly used in fluorescence microscopy, immunohistochemistry, flow cytometry, and other fluorescence-based assays.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 605 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	95.959 µL	479.796 µL	959.592 µL	4.798 mL	9.596 mL
5 mM	19.192 µL	95.959 µL	191.918 µL	959.592 µL	1.919 mL
10 mM	9.596 µL	47.98 µL	95.959 µL	479.796 µL	959.592 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1042.11 g/mol	=	mL	x	mM	=	mmol

References

View all 40 references: Citation Explorer

Click-iT ® Plus OPP Alexa Fluor ® Protein Synthesis Assay in Embryonic Cells.

Authors:

Li, Yan and Ji, Xu and Chang, Lu and Tang, Jianan and Hua, Min-Min and Liu, Jing and O'Neill, Christopher and Huang, Xuefeng and Jin, Xingliang

Journal:

Bio-protocol (2022): e4441

Visualization of preimplantation uterine fluid absorption in mice using Alexa Fluor™ 488 hydrazide.

Authors:

Li, Yuehuan and Martin, Taylor Elijah and Hancock, Jonathan Matthew and Li, Rong and Viswanathan, Suvitha and Lydon, John P and Zheng, Yi and Ye, Xiaoqin

Journal:

Biology of reproduction (2022)

Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.

Authors:

Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu

Journal:

Neural regeneration research (2022): 2316-2320

Molecular and Spectroscopic Characterization of Green and Red Cyanine Fluorophores from the Alexa Fluor and AF Series.

Authors:

Gebhardt, Christian and Lehmann, Martin and Reif, Maria M and Zacharias, Martin and Gemmecker, Gerd and Cordes, Thorben

Journal:

Chemphyschem : a European journal of chemical physics and physical chemistry (2021): 1546

Evaluation of Blood-Brain Barrier Integrity Using Vascular Permeability Markers: Evans Blue, Sodium Fluorescein, Albumin-Alexa Fluor Conjugates, and Horseradish Peroxidase.

Authors:

Ahishali, Bulent and Kaya, Mehmet

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 87-103

Physical properties

Molecular weight	1042.11
Solvent	DMSO

Spectral properties

Excitation (nm)	603
Emission (nm)	623

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 31, 2026