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iFluor® 555 goat anti-mouse IgG (H+L)

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 555 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ~559 nm and ~569 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 555 goat anti-mouse IgG (H+L) conjugate (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 350 goat anti-mouse IgG (H+L)3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L)4034273700010.9110.480.77
iFluor® 488 goat anti-mouse IgG (H+L)4915167500010.910.210.11
iFluor® 514 goat anti-mouse IgG (H+L)5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L)5375609000010.6810.260.16
iFluor® 594 goat anti-mouse IgG (H+L)58760320000010.5310.050.04
iFluor® 633 goat anti-mouse IgG (H+L)64065425000010.2910.0620.044
iFluor® 647 goat anti-mouse IgG (H+L)65667025000010.2510.030.03
iFluor® 680 goat anti-mouse IgG (H+L)68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L)69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L)75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L)78781225000010.1310.10.09
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*3454502000010.9510.830.23
iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*4034273700010.9110.480.77
iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*4915167500010.910.210.11
iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*5115277500010.8310.2650.116
iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*5375609000010.6810.260.16
iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*58760320000010.5310.050.04
iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*64065425000010.2910.0620.044
iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*65667025000010.2510.030.03
iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*68470122000010.2310.0970.094
iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*69071322000010.2310.090.04
iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*75777927500010.1210.0440.039
iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*78781225000010.1310.10.09
iFluor® 555 goat anti-rabbit IgG (H+L)55757010000010.6410.230.14
iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*55757010000010.6410.230.14
iFluor® 546 goat anti-mouse IgG (H+L)54155710000010.6710.250.15
iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*54155710000010.6710.250.15
iFluor® 568 goat anti-mouse IgG (H+L)56858710000010.5710.340.15
iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*56858710000010.5710.340.15
iFluor® 800 goat anti-mouse IgG (H+L) 80182025000010.1110.030.08
iFluor® 800 goat anti-mouse IgG (H+L) *Cross Adsorbed*80182025000010.1110.030.08
iFluor® 810 goat anti-mouse IgG (H+L) 81182225000010.0510.090.15
iFluor® 810 goat anti-mouse IgG (H+L) *Cross Adsorbed*81182225000010.0510.090.15
iFluor® 820 goat anti-mouse IgG (H+L)82285025000010.110.16
iFluor® 820 goat anti-mouse IgG (H+L) *Cross Adsorbed*82285025000010.110.16
iFluor® 840 goat anti-mouse IgG (H+L)8368792000001-0.20.09
iFluor® 840 goat anti-mouse IgG (H+L) *Cross Adsorbed*8368792000001-0.20.09
iFluor® 860 goat anti-mouse IgG (H+L)85387825000010.10.14
iFluor® 860 goat anti-mouse IgG (H+L) *Cross Adsorbed*85387825000010.10.14
Show More (31)

Citations

View all 7 citations: Citation Explorer
Curcumin inhibits the pruritus in mice through mast cell MrgprB2 receptor
Authors: Jiang, Yucui and Zong, Yingxin and Du, Ying and Zhang, Miaomiao and Ye, Fan and Zhang, Jian and Yang, Yan and Zhu, Chan and Tang, Zongxiang
Journal: Inflammation Research (2023): 1--13
The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells
Authors: Zhang, Xiaobei and Jin, Meng and Liu, Shiqi and Zang, Mingde and Hu, Lei and Du, Tao and Zhang, Baogui
Journal: Heliyon (2023)
{\.I}nd{\"u}klenmi{\c{s}} pluripotent k{\"o}k h{\"u}cre tabanl{\i} multiple skleroz modelinde oligodendrositlere {\"o}zg{\"u} fenotiplerin ara{\c{s}}t{\i}r{\i}lmas{\i}
Authors: Kurt Vatanda{\c{s}}lar, Burcu
Journal: (2018)
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)
Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo
Journal: Tumor Biology (2016): 1--12

References

View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
Authors: Buckova A., undefined
Journal: Epidemiol Mikrobiol Imunol (2010): 133
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
Authors: Wu W, Yi P, He P, Jing T, Liao K, Yang K, Wang H.
Journal: Anal Chim Acta (2010): 126
Page updated on October 28, 2024

Ordering information

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Unit size
200 ug
1 mg
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Physical properties

Molecular weight

~150000

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.14

Extinction coefficient (cm -1 M -1)

1000001

Excitation (nm)

557

Emission (nm)

570

Quantum yield

0.641

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup> 555 goat anti-mouse IgG conjugate (Red, Right) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>&reg;</sup> 555&nbsp; (Red, left), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup> 555 goat anti-mouse IgG conjugate (Red, Right) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>&reg;</sup> 555&nbsp; (Red, left), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
HeLa cells were incubated with mouse anti-tubulin followed by AAT&rsquo;s iFluor<sup>TM</sup> 555 goat anti-mouse IgG conjugate (Red, Right) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>&reg;</sup> 555&nbsp; (Red, left), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
HeLa cells&nbsp;were stained&nbsp;with (Tubulin+) or without (Tubulin-) mouse anti-tubulin and then&nbsp;visualized&nbsp;with iFluor® 594&nbsp;goat anti-mouse IgG (Right) or Alexa Fluor&reg; 594 goat anti-mouse IgG (Left).