iFluor® 820 goat anti-mouse IgG (H+L)

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 532-561, 633-647, and 808 nm). iFluor® 820 goat anti-mouse IgG (H+L) conjugate has fluorescence excitation and emission maxima of ∼822 nm and ∼850 nm, respectively. These unique spectral characteristics makes iFluor® 820 goat anti-mouse IgG (H+L) conjugates ideal for various NIR imaging applications, including Western blotting, ELISA, protein arrays, tissue section imaging, and in vivo imaging.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
iFluor® 350 goat anti-mouse IgG (H+L)	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 goat anti-mouse IgG (H+L)	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 goat anti-mouse IgG (H+L)	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 514 goat anti-mouse IgG (H+L)	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 goat anti-mouse IgG (H+L)	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 555 goat anti-mouse IgG (H+L)	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 goat anti-mouse IgG (H+L)	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 633 goat anti-mouse IgG (H+L)	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 goat anti-mouse IgG (H+L)	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 goat anti-mouse IgG (H+L)	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 goat anti-mouse IgG (H+L)	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 goat anti-mouse IgG (H+L)	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 goat anti-mouse IgG (H+L)	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 350 goat anti-mouse IgG (H+L) Cross Adsorbed	345	450	20000¹	0.95¹	0.83	0.23
iFluor® 405 goat anti-mouse IgG (H+L) Cross Adsorbed	403	427	37000¹	0.91¹	0.48	0.77
iFluor® 488 goat anti-mouse IgG (H+L) Cross Adsorbed	491	516	75000¹	0.9¹	0.21	0.11
iFluor® 514 goat anti-mouse IgG (H+L) Cross Adsorbed	511	527	75000¹	0.83¹	0.265	0.116
iFluor® 532 goat anti-mouse IgG (H+L) Cross Adsorbed	537	560	90000¹	0.68¹	0.26	0.16
iFluor® 555 goat anti-mouse IgG (H+L) Cross Adsorbed	557	570	100000¹	0.64¹	0.23	0.14
iFluor® 594 goat anti-mouse IgG (H+L) Cross Adsorbed	587	603	200000¹	0.53¹	0.05	0.04
iFluor® 633 goat anti-mouse IgG (H+L) Cross Adsorbed	640	654	250000¹	0.29¹	0.062	0.044
iFluor® 647 goat anti-mouse IgG (H+L) Cross Adsorbed	656	670	250000¹	0.25¹	0.03	0.03
iFluor® 680 goat anti-mouse IgG (H+L) Cross Adsorbed	684	701	220000¹	0.23¹	0.097	0.094
iFluor® 700 goat anti-mouse IgG (H+L) Cross Adsorbed	690	713	220000¹	0.23¹	0.09	0.04
iFluor® 750 goat anti-mouse IgG (H+L) Cross Adsorbed	757	779	275000¹	0.12¹	0.044	0.039
iFluor® 790 goat anti-mouse IgG (H+L) Cross Adsorbed	787	812	250000¹	0.13¹	0.1	0.09
iFluor® 546 goat anti-mouse IgG (H+L)	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 546 goat anti-mouse IgG (H+L) Cross Adsorbed	541	557	100000¹	0.67¹	0.25	0.15
iFluor® 568 goat anti-mouse IgG (H+L)	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 568 goat anti-mouse IgG (H+L) Cross Adsorbed	568	587	100000¹	0.57¹	0.34	0.15
iFluor® 800 goat anti-mouse IgG (H+L)	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 800 goat anti-mouse IgG (H+L) Cross Adsorbed	801	820	250000¹	0.11¹	0.03	0.08
iFluor® 810 goat anti-mouse IgG (H+L)	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 810 goat anti-mouse IgG (H+L) Cross Adsorbed	811	822	250000¹	0.05¹	0.09	0.15
iFluor® 840 goat anti-mouse IgG (H+L)	836	879	200000¹	-	0.2	0.09
iFluor® 840 goat anti-mouse IgG (H+L) Cross Adsorbed	836	879	200000¹	-	0.2	0.09
iFluor® 860 goat anti-mouse IgG (H+L)	853	878	250000¹		0.1	0.14
iFluor® 860 goat anti-mouse IgG (H+L) Cross Adsorbed	853	878	250000¹		0.1	0.14
iFluor® 820 goat anti-rabbit IgG (H+L)	822	850	250000¹		0.11	0.16
iFluor® 820 goat anti-rabbit IgG (H+L) Cross Adsorbed	822	850	250000¹		0.11	0.16
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Page updated on December 17, 2024

Ordering information

Price
Unit size	200 ug 1 mg
Catalog Number	4800848009
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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction Factor (260 nm)	0.11
Correction Factor (280 nm)	0.16
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	822
Emission (nm)	850

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501