Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe
Trizma® is a proprietary chemical buffer used similarly to Tris buffer. It is commonly used in protein extraction for many types of IHC assays as well as blot applications. It is used in sandwich ELISA protocols for protection of the antibodies, and in electrophoresis of nucleic acids.
To prepare L of Trizma® Buffer (pH 7.0 to 9.2):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Current molarity: 0.1 M | Adjust the molarity of the solution by using the slider below |
Current pH: 9 | Adjust the pH of the solution by using the slider below |
Table 1. Required components
Component | Amount | Concentration |
Trizma Hydrochloride (mw: 157.60 g/mol) | 1.011 g | 0.006415 M |
Trizma Base (mw: 121.14 g/mol) | 11.337 g | 0.09358 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 1.011 g of Trizma Hydrochloride to the solution.
- Add 11.337 g of Trizma Base to the solution.
- Add distilled water until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe." AAT Bioquest, Inc., 23 Dec. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0. | |
APA | AAT Bioquest, Inc. (2024, December 23). Quest Calculate™ Trizma® Buffer (pH 7.0 to 9.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0. | |
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