MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe
MOPSO buffer is one of the Good's zwitterionic buffer with an approximate pH range of 6.5-7.9. It has a good buffering capacity but does react with iron ions. MOPSO is used for a variety of assays that require physiological pH.
To prepare L of MOPSO Buffer (0.5 M, 6.9 pH):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
MOPSO, Free acid (mw: 225.26 g/mol) | 112.63 g | 0.5 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 112.63 g of MOPSO, Free acid to the solution.
- Adjust solution to desired pH by 10N NaOH.
- Add dH2O until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe." AAT Bioquest, Inc., 31 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9. | |
APA | AAT Bioquest, Inc. (2024, October 31). Quest Calculate™ MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9. | |
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