Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe
Potassium phosphate buffers, sometimes called Gomori buffers, consist of a mixture of monobasic dihydrogen phosphate and dibasic monohydrogen phosphate. These buffers have excellent buffering capacity and are highly soluble in water. However, potassium phosphate buffer reacts with some metal ions including calcium and magnesium, and inhibits enzymatic reactions. Additionally, all phosphates precipitate in ethanol, which can be deleterious to some nucleic acid preparations that typically use ethanol solutions. Potassium phosphate buffer is used in research fields ranging from molecular biology to botany, due to its adaptability and affordability.
To prepare L of Potassium Phosphate (pH 5.8 to 8.0):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Current molarity: 0.1 M | Adjust the molarity of the solution by using the slider below |
Current pH: 8 | Adjust the pH of the solution by using the slider below |
Table 1. Required components
Component | Amount | Concentration |
Potassium phosphate dibasic (mw: 174.18 g/mol) | 16.282 g | 0.09348 M |
Potassium Phosphate Monobasic (mw: 136.09 g/mol) | 887.8 mg | 0.006523 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 16.282 g of Potassium phosphate dibasic to the solution.
- Add 887.8 mg of Potassium Phosphate Monobasic to the solution.
- Add dH2O until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe." AAT Bioquest, Inc., 31 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-ph-5-8-to-8-0. | |
APA | AAT Bioquest, Inc. (2024, October 31). Quest Calculate™ Potassium Phosphate (pH 5.8 to 8.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-ph-5-8-to-8-0. | |
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This online tool has been cited in 1 publications, including
Detailed characterization of the cell wall structure and composition of nordic green microalgae
Authors:
Journal: Journal of agricultural and food chemistry (2022): 9711--9721
Authors:
Journal: Journal of agricultural and food chemistry (2022): 9711--9721