Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe

Commonly used for various immunoassay applications and for many protein and antibody conjugation procedures, including sandwich ELISA, which require experimental surface coatings. Carbonate-bicarbonate buffer is used extensively in molecular and cell biology, biochemistry, and in the medical field, where it is the most commonly used small intestine buffer. It has good buffering capacity and is easy to prepare, with excellent shelf life.

To prepare

L of Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6):

Change the value in the textbox above to scale the recipe volume

Current molarity: 0.1	Adjust the molarity of the solution by using the slider below
Current pH: 10.6	Adjust the pH of the solution by using the slider below

Table 1. Required components

Component	Amount	Concentration
Sodium bicarbonate (mw: 84.01 g / mol)	#	#
Sodium carbonate (anhydrous) (mw: 105.99 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium bicarbonate to the solution
Add # of Sodium carbonate (anhydrous) to the solution
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe." AAT Bioquest, Inc., 21 Sep. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6.
APA	AAT Bioquest, Inc. (2025, September 21). Quest Calculate™ Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/carbonate-bicarbonate-buffer-ph-9-2-to-10-6.
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This online resource has been cited in 1 publications, including

Simultaneous Determination of Lidocaine and Bupivacaine in Human Saliva Using Gas Chromatography-Mass Spectrometry

Authors:

Dubey, Pallavi and Mohammed, Idris

Journal:

Arab Journal of Forensic Sciences \& Forensic Medicine (2024): 19--28