Extracellular Saline (1x, pH 7.3) Preparation and Recipe
To prepare L of Extracellular Saline (1x, pH 7.3):
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Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Sodium chloride (mw: 58.44 g/mol) | 8.182 g | 0.14 M |
Potassium Chloride (mw: 74.55 g/mol) | 0.402 g | 0.0054 M |
Calcium Chloride (mw: 110.98 g/mol) | 0.294 g | 0.002 M |
Magnesium sulfate (mw: 120.37 g/mol) | 1.204 g | 0.01 M |
Hepes (mw: 238.30 g/mol) | 1.191 g | 0.005 M |
Glucose (mw: 180.16 g/mol) | 1.802 g | 0.01 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 8.182 g of Sodium chloride to the solution.
- Add 0.402 g of Potassium Chloride to the solution.
- Add 0.294 g of Calcium Chloride to the solution.
- Add 1.204 g of Magnesium sulfate to the solution.
- Add 1.191 g of Hepes to the solution.
- Add 1.802 g of Glucose to the solution.
- Add distilled water until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Extracellular Saline (1x, pH 7.3) Preparation and Recipe." AAT Bioquest, Inc., 30 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/extracellular-saline-rnase-free-1x-ph-7-3. | |
APA | AAT Bioquest, Inc. (2024, October 30). Quest Calculate™ Extracellular Saline (1x, pH 7.3) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/extracellular-saline-rnase-free-1x-ph-7-3. | |
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