Potassium Phosphate Buffer (1 M, pH 6.5) Preparation and Recipe

Potassium phosphate buffers, sometimes called Gomori buffers, consist of a mixture of monobasic dihydrogen phosphate and dibasic monohydrogen phosphate. These buffers have excellent buffering capacity and are highly soluble in water. However, potassium phosphate buffer reacts with some metal ions including calcium and magnesium, and inhibits enzymatic reactions. Additionally, all phosphates precipitate in ethanol, which can be deleterious to some nucleic acid preparations that typically use ethanol solutions. Potassium phosphate buffer is used in research fields ranging from molecular biology to botany, due to its adaptability and affordability.

To prepare L of Potassium Phosphate Buffer (1 M, pH 6.5):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Potassium Phosphate Monobasic (mw: 136.09 g/mol)	95 g	0.698 M
Potassium phosphate dibasic (mw: 174.18 g/mol)	52.5 g	0.3014 M

Prepare 800 mL of distilled water in a suitable container.
Add 95 g of Potassium Phosphate Monobasic to the solution.
Add 52.5 g of Potassium phosphate dibasic to the solution.
Adjust the pH to 6.5.
Add distilled water until the volume is 1 L.
Filter sterilize and store at room temperature.

Physiological Buffer

pH Buffering

Sample Preparation

BioAssays

Misc

Cell/Culture/Growth Media

Gel Electrophoresis

Name ▲ ▼
Extracellular Saline (1x, pH 7.3)
HBSS (Hank's Balanced Salt Solution)
HEPES Buffer (1 M, 7.5 pH)
HHBS (Hank's Buffer with Hepes)
PBS (Phosphate Buffered Saline) (1X, pH 7.4)
PBS with Ca2+and Mg2+ (D-PBS)
Tyrode's Solution, Acidic

Name ▲ ▼
ACES Buffer (0.1 M, 6.7 pH)
Acetate Buffer (0.1 M, pH 5.0)
Acetate Buffer (pH 3.6 to 5.6)
ADA Buffer (0.5 M, 6.6 pH)
Ammonium Acetate
Ammonium Bicarbonate (50 mM, pH 7.8)
Ammonium Sulfate, Saturated
BES-Buffered Saline (2X) (0.05 M, pH 6.95)
Bicine (1 M, pH 8.26)
Bis-Tris Buffer (1 M, 6.5 pH)
Borate Buffer
CAPS (0.5 M, pH 10.4)
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)
CHES (0.5 M, pH 9.5)
Citrate Buffer (0.1 M, pH 6.0)
Citrate Buffer (pH 3.0 to 6.2)
Citrate-Phosphate Buffer (0.15 M, pH 5.0)
Citrate-Phosphate Buffer (110 mM, pH 5.6)
Citrate-Phosphate Buffer (40 mM, pH 5.6)
Diethanolamine (1 M, pH 9.8)
EBSS (magnesium, calcium, phenol red) (pH 7.0)
Glycine (0.1 M, pH 2.2)
Glycine-HCl Buffer (0.1 M, pH 3.0)
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)
HEPPSO (1 M, pH 7.85)
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)
Imidazole-HCl Buffer (0.05 M, pH 7.0)
Maleic Acid (1 M, pH 7.5)
MES (0.5 M, pH 6)
MOPS Buffer (10X) (0.2 M, pH 7)
MOPSO Buffer (0.5 M, 6.9 pH)
Phosphate Buffer (pH 5.8 to 7.4)
PIPES Buffer (1 M, 6.8 pH)
Potassium Phosphate Buffer (1 M, pH 6.5)
Potassium Phosphate (pH 5.8 to 8.0)
Sodium Acetate (3 M, pH 5.2)
Sodium Borate Buffer (1 M, pH 8.5)
Sodium Phosphate Solution
SSC buffer (20X) (3 M, pH 7)
TAE (1 M, pH 8.6)
TB Buffer
TBS (1 M, pH 7.4)
TES Buffer (1 M, 7.5 pH)
Tricine (1 M, pH 8.05)
Tris Buffer (1 M, pH 7.2)
Tris-Buffered Saline (TBS, 0.1 M)
Tris-citrate Buffer (pH 8.0)
Trizma® Buffer (pH 7.0 to 9.2)

Name ▲ ▼
ACK Lysis Buffer
Alkaline Sodium Thiosulfate
BAC DNA Microinjection Buffer
DEAE-dextran
EDTA Solution
Elution Buffer
Gey's Balanced Salt Solution
H-50 Buffer
Lysis Solution
Osmium Tetroxide Solution
Paraformaldehyde Solution (8%)
TE Buffer 10X
Trichloroacetic Acid (TCA)

Name ▲ ▼
Anesthetic (MS222) Solution
Bradford Stock Solution
5-Fluorocytosine (5-FC) Solution
Chloroquine
Cresol Red Solution
Ethidium Bromide Solution
Immunofluorescence Quenching Solution
Mayer's Hemalum
Nuclear Fast Red Solution
Pyronin Y (PY) Solution
SSC (Northern Blot, 20x)
SSPE (Blot, 20x, pH 7.4)

Name ▲ ▼
Dithiothreitol (DTT) (1 M)
Sodium Iodide/Sodium Sulfite Solution

Name ▲ ▼
Bath Solution (Ca2+/Mg2+ Free)
Calcium Chloride Stock Solution
Carbenicillin Solution
Chelation Medium
Digestion Medium
Embryonic Medium
IPTG Solution
M9 Minimal Salts
Modified Barth's Saline (MBS)
Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix
Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes

Name ▲ ▼
Acrylamide Stock Solution
Electrode Buffer
Electrophoretic Transfer Buffer (20x)
Imidazole-SDS Solution
SDS-PAGE SDS Running Buffer (10x)
Sodium Carbonate Transfer Buffer (40x, pH 9.5)
Tris/EACA Buffer
Tris-Glycine Transfer Buffer (20x)

References

This online tool may be cited as follows

MLA	"Quest Calculate™ Potassium Phosphate Buffer (1 M, pH 6.5) Preparation and Recipe." AAT Bioquest, Inc., 23 Nov. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-buffer-1-m-ph-6-5.
APA	AAT Bioquest, Inc. (2024, November 23). Quest Calculate™ Potassium Phosphate Buffer (1 M, pH 6.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/potassium-phosphate-buffer-1-m-ph-6-5.
BibTeX	EndNote	RefMan

Potassium Phosphate Buffer (1 M, pH 6.5) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA