TE Buffer 10X Preparation and Recipe
TE buffer is used as a protective measure against DNA and RNA degredation, storing the two molecules and maintaining proper pH levels.
To prepare L of TE Buffer 10X:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Tris-Cl (desired pH) (mw: 157.594 g/mol) | 15.759 g | 0.1 M |
EDTA (pH 8) (mw: 292.24 g/mol) | 2.92 g | 0.01 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 15.759 g of Tris-Cl (desired pH) to the solution.
- Add 2.92 g of EDTA (pH 8) to the solution.
- Add distilled water until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ TE Buffer 10X Preparation and Recipe." AAT Bioquest, Inc., 31 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/te-buffer-10x. | |
APA | AAT Bioquest, Inc. (2024, October 31). Quest Calculate™ TE Buffer 10X Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/te-buffer-10x. | |
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