Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe
Imidazole is a mild buffer that is useful for IHC applications in multiple research areas such as biochemistry and molecular biology. It has a good shelf-life and is well-tolerated by multiple cell types.
To prepare L of Imidazole-HCl Buffer (0.05 M, pH 7.0):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Imidazole (mw: 68.08 g/mol) | 3.404 g | 0.05 M |
Hydrochloric acid (mw: 36.46 g/mol) | 0.886 g | 0.0243 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 3.404 g of Imidazole to the solution.
- Add 0.886 g of Hydrochloric acid to the solution.
- Adjust solution to desired pH using HCl (typically pH ≈ 7.0).
- Add distilled water until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe." AAT Bioquest, Inc., 31 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/imidazole-hcl-buffer-ph-7-0. | |
APA | AAT Bioquest, Inc. (2024, October 31). Quest Calculate™ Imidazole-HCl Buffer (0.05 M, pH 7.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/imidazole-hcl-buffer-ph-7-0. | |
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