Embryonic Medium Preparation and Recipe
Embryonic medium is used for culturing embryos.
To prepare L of Embryonic Medium:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Sodium chloride (mw: 58.44 g/mol) | 0.87 g | 0.015 M |
Potassium Chloride (mw: 74.55 g/mol) | 0.04 g | 0.0005 M |
Calcium chloride dihydrate (mw: 147.01 g/mol) | 0.15 g | 0.001 M |
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol) | 0.25 g | 0.001 M |
Potassium Phosphate Monobasic (mw: 136.09 g/mol) | 0.02 g | 0.00015 M |
Sodium phosphate dibaic dodecahydrate (mw: 358.14 g/mol) | 0.02 g | 0.00005 M |
Sodium bicarbonate (mw: 84.01 g/mol) | 0.06 g | 0.0007 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 0.87 g of Sodium chloride to the solution.
- Add 0.04 g of Potassium Chloride to the solution.
- Add 0.15 g of Calcium chloride dihydrate to the solution.
- Add 0.25 g of Magnesium Sulfate Heptahydrate to the solution.
- Add 0.02 g of Potassium Phosphate Monobasic to the solution.
- Add 0.02 g of Sodium phosphate dibaic dodecahydrate to the solution.
- Add 0.06 g of Sodium bicarbonate to the solution.
- Add distilled water until the volume is 1 L.
- Adjust the pH to 7.0-7.5.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Embryonic Medium Preparation and Recipe." AAT Bioquest, Inc., 31 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/embryonic-medium. | |
APA | AAT Bioquest, Inc. (2024, October 31). Quest Calculate™ Embryonic Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/embryonic-medium. | |
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