ATTO 633 acid

• Ex/Em: 629/651 nm
• Extinction coefficient: 130,000 cm^-1M^-1
• High Quantum Yield: Offers bright fluorescence, ideal for sensitive detection in various imaging techniques
• Exceptional Stability: Maintains fluorescence under prolonged light exposure, temperature variations, and across a wide pH range (2–11)
• Broad Applications: Suited for high-precision techniques such as single-molecule detection and super-resolution microscopy (PALM, dSTORM, STED)

ATTO 633 is a bright-red fluorescent dye, characterized by its strong absorption, high fluorescence quantum yield, and exceptional photo and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 610-645 nm wavelength range, compatible with both the 633 nm line of the He-Ne laser and the 635 nm line of the diode laser. The dye maintains stable fluorescence over a wide pH range (2-11), allowing for its use in diverse experimental conditions. Upon conjugation to a substrate, ATTO 633 becomes cationic, carrying a net positive charge of +1. These properties make ATTO 633 particularly suitable for high-precision applications, including single-molecule detection and super-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 633 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 633 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.