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ATTO 514 acid
Product key features
- Ex/Em: 510/531 nm
- Extinction coefficient: 115,000 cm-1M-1
- Robust Quantum Yield: Delivers bright fluorescence, ideal for sensitive detection across various imaging techniques
- Exceptional Stability: Offers high photostability and thermal stability
- Excellent Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Product description
ATTO 514 is a rhodamine-based fluorophore characterized by exceptional water solubility, strong absorption, high fluorescence quantum yield, and outstanding thermal and photo-stability. This makes it highly suitable for applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, it finds utility in flow cytometry (FACS), fluorescence in-situ hybridization (FISH), and various scientific methodologies. ATTO 514 is hydrophilic and exhibits optimal fluorescence efficiency when excited within the 510-535 nm range, making it an ideal match for the 514 nm line of an Argon-Ion laser.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| ATTO 488 acid | 499 | 520 | 90000 | 0.80 | 0.22 | 0.09 |
| ATTO 532 acid | 531 | 552 | 115000 | 0.90 | 0.22 | 0.11 |
| ATTO 647 acid | 646 | 666 | 120000 | 0.20 | 0.08 | 0.04 |
| ATTO 647N acid | 645 | 663 | 150000 | 0.651 | 0.06 | 0.05 |
| ATTO 594 acid | 602 | 621 | 120000 | 0.85 | 0.26 | 0.51 |
| ATTO 565 acid | 562 | 589 | 120000 | 0.90 | 0.27 | 0.12 |
| ATTO 390 acid | 390 | 475 | 24000 | .90 | 0.46 | 0.09 |
| ATTO 425 acid | 438 | 484 | 45000 | 0.90 | 0.19 | 0.17 |
| ATTO 495 acid | 497 | 525 | 80000 | 0.2 | 0.45 | 0.37 |
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References
View all 7 references: Citation Explorer
The new live imagers MitoMM1/2 for mitochondrial visualization.
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
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