ATTO 647N acid

• Ex/Em: 645/663 nm
• Extinction Coefficient: 150,000 cm⁻¹M⁻¹
• Robust Quantum Yield: Delivers bright fluorescence, ideal for sensitive detection across various imaging techniques
• Exceptional Stability: Offers high photostability, thermal stability, and pH insensitivity across a broad range (2–11)
• Ozone-Resistant: Enhanced resistance to atmospheric ozone degradation ensures reliable performance in microarray applications

ATTO 647N is a rhodamine-based fluorescent dye optimized for use in the red spectral region, with similar spectral characteristics as Cy5. It is characterized by high molar absorptivity, a strong fluorescence quantum yield, and excellent thermal and photostability. The dye is moderately hydrophilic and exhibits optimal excitation within the 625-660 nm range, making it compatible with the 647 nm line of Krypton-Ion lasers and the 650 nm line of diode lasers. ATTO 647N maintains stable fluorescence across a broad pH range (pH 2-11), supporting its application under diverse experimental conditions. Upon conjugation to a substrate, the dye becomes cationic, carrying a net positive charge of +1. Unlike cyanine dyes, ATTO 647N demonstrates enhanced resistance to atmospheric ozone degradation, which increases its reliability in microarray applications. ATTO 647N is particularly effective for high-precision applications such as single-molecule detection, super-resolution microscopy techniques (e.g., SIM and STED), flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 647N acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 647N conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.