ATTO 647 acid

• Ex/Em: 646/666 nm
• Extinction coefficient: 120,000 cm^-1M^-1
• Robust Quantum Yield: Offers bright fluorescence, making it suitable for sensitive detection in various imaging techniques
• High Stability: Ensures consistent fluorescence with excellent photostability and thermal stability
• Excellent Hydrophilicity: Readily dissolves in aqueous environments for optimal performance in biological assays
• Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy

ATTO 647 is a rhodamine-derived fluorescent dye optimized for applications within the red spectral region, offering spectral characteristics similar to Cy5. Its characterized by a high molar absorptivity, robust fluorescence quantum yield, high photostability, and good aqueous solubility, making it well-suited for demanding experimental conditions. The dye is highly hydrophilic, with an excitation maximum between the 615-660 nm range, making it compatible with the 633 nm He:Ne laser, 647 nm Krypton-Ion laser, and 650 nm diode laser. As a zwitterionic molecule, ATTO 647 carries a net neutral charge. The dye is stable under physiological pH conditions and in buffers with a pH of up to 8, though it gradually degrades at higher pH levels. ATTO 647 is ideal for advanced applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays.

ATTO 647 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 647 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.