ATTO 700 acid

• Ex/Em: 699/715 nm
• Extinction Coefficient: 120,000 cm⁻¹M⁻¹
• Robust Quantum Yield: Delivers bright fluorescence, ideal for sensitive detection across various imaging techniques
• Exceptional Stability: Offers high photostability and thermal stability for sensitive applications
• Zwitterionic: Minimizes non-specific binding to biological surfaces, reducing background noise in live-cell imaging and super-resolution microscopy

ATTO 700 is a near-infrared fluorescent dye characterized by its strong absorption, high photo and thermal stability, and good aqueous solubility. It is optimally excited within the 670-715 nm wavelength range. As a zwitterionic compound, ATTO 700 remains electrically neutral when conjugated to biomolecules or other substrates. Its strong electron-accepting properties result in efficient fluorescence quenching by electron donors such as guanine and tryptophan. These properties make ATTO 700 ideal for precise applications including single-molecule detection and super-resolution microscopy techniques like PALM, dSTORM, and STED. Furthermore, ATTO 700 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a variety of other biological assays, making it a versatile tool in advanced fluorescence-based research.

ATTO 700 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 700 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.