ATTO 620 acid

• Ex/Em: 619/641 nm
• Extinction coefficient: 120,000 cm^-1M^-1
• High Quantum Yield: Offers bright fluorescence, making it suitable for sensitive detection in various imaging techniques
• Exceptional Stability: Maintains fluorescence under prolonged light exposure, temperature variations, and across a wide pH range (2–11)
• Temperature-Dependent Fluorescence: Enables precise thermal mapping in live cells, protein studies, and reaction monitoring

ATTO 620 is a bright-red fluorescent dye, characterized by its strong absorption, exceptional photo and thermal stability, and temperature-dependent fluorescence. It demonstrates moderate hydrophilicity and optimal excitation within the 600-630 nm wavelength range. The dye maintains stable fluorescence over a wide pH range (2-11), allowing for its use in diverse experimental conditions. When conjugated to a substrate, ATTO 620 becomes cationic, carrying a net positive charge of +1. These properties make ATTO 620 particularly suitable for high-precision applications, including single-molecule detection and super-resolution microscopy techniques such as PALM, dSTORM, and STED. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 620 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 620 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.