ATTO 610 acid

• Ex/Em: 615/632 nm
• Extinction coefficient: 150,000 cm^-1M^-1
• High Quantum Yield: Offers bright fluorescence, making it suitable for sensitive detection in various imaging techniques
• Exceptional Stability: Maintains fluorescence under prolonged light exposure and remains stable across temperature variations
• Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

ATTO 610 is a carbopyronin-based fluorescent dye known for its strong absorption, high fluorescence quantum yield, and exceptional photostability and thermal stability. It is moderately hydrophilic and optimally excited at wavelengths between 595 and 625 nm. Upon coupling to a substrate, ATTO 610 becomes cationic, carrying a net electrical charge of +1. The dye remains stable under physiological pH conditions and in buffers with a pH of up to 8, though it gradually degrades at higher pH levels. ATTO 610 is ideal for advanced applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays.

ATTO 610 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 610 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.