ATTO 565 acid

Product key features

Ex/Em: 562/589 nm
Extinction coefficient: 120,000 cm^-1M^-1
High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
Enhanced Signal Separation: Provides effective signal clarity by minimizing background interference
Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

Product description

ATTO 565 acid is a commonly used amine-reactive rhodamine dye. It might be used for labeling peptides, proteins and other amino-containing compounds such as amino-modified oligos. It reacts with compounds containing amino groups in the presence of a coupling reagent such as EDC. It forms a chemically stable amide bond between the dye and amino compounds. ATTO 565 acid has strong absorption, high fluorescence quantum yield and high thermal/photostability. However, it has low water solubility. For the applications that require high water solubility, you may consider iFluor® 568 acid.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
ATTO 488 acid	499	520	90000	0.80	0.22	0.09
ATTO 532 acid	531	552	115000	0.90	0.22	0.11
ATTO 647 acid	646	666	120000	0.20	0.08	0.04
ATTO 647N acid	645	663	150000	0.65¹	0.06	0.05
ATTO 594 acid	602	621	120000	0.85	0.26	0.51
ATTO 514 acid	510	531	115,000	0.85	0.21	0.08
ATTO 390 acid	390	475	24000	.90	0.46	0.09
ATTO 425 acid	438	484	45000	0.90	0.19	0.17
ATTO 495 acid	497	525	80000	0.2	0.45	0.37
ATTO 550 acid	553	574	120000	0.80	0.23	0.10
ATTO 590 acid	592	621	120000	0.80	0.39	0.43
ATTO 610 acid	615	632	150000	0.70	0.03	0.06
ATTO 620 acid	619	641	120000¹	0.5¹	0.04	0.06
ATTO 633 acid	629	651	130000	0.64¹	0.04	0.05
ATTO 655 acid	661	679	125000	0.3¹	0.24	0.08
ATTO 680 acid	679	696	125000	0.30	0.30	0.17
ATTO 700 acid	699	715	120000	0.25	0.26	0.41
ATTO 565 TCO	562	589	120000	0.90	0.27	0.12
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References

View all 7 references: Citation Explorer

The new live imagers MitoMM1/2 for mitochondrial visualization.
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54

DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053

Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364

Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925

The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95

Page updated on November 23, 2024

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Catalog Number	2827
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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	624.61
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.27
Correction Factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	120000
Excitation (nm)	562
Emission (nm)	589
Quantum yield	0.90