ATTO 590 acid

• Ex/Em: 592/621 nm
• Extinction coefficient: 120,000 cm^-1M^-1
• High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
• Compatibility with Quenchers: Fluorescence effectively quenched by BHQ®-2 dye, allowing for precise FRET applications and molecular beacon assays
• Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

ATTO 590 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent photostability and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 575-610 nm wavelength range. ATTO 590 emits in the orange-red region of the visible spectrum, with fluorescence effectively quenched by BHQ®-2 dye. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. ATTO 590 is a suitable alternative to Alexa Fluor® 594 for these applications.

ATTO 590 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 590 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.