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ATTO 590 acid

Product key features

  • Ex/Em: 592/621 nm
  • Extinction coefficient: 120,000 cm-1M-1
  • High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
  • Compatibility with Quenchers: Fluorescence effectively quenched by BHQ®-2 dye, allowing for precise FRET applications and molecular beacon assays
  • Ideal for Super-Resolution Imaging: Suited for PALM, dSTORM, and STED techniques in high-resolution microscopy

Product description

ATTO 590 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent photostability and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 575-610 nm wavelength range. ATTO 590 emits in the orange-red region of the visible spectrum, with fluorescence effectively quenched by BHQ®-2 dye. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. ATTO 590 is a suitable alternative to Alexa Fluor® 594 for these applications.

ATTO 590 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing ATTO 590 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 488 acid499520900000.800.220.09
ATTO 532 acid5315521150000.900.220.11
ATTO 647 acid6466661200000.200.080.04
ATTO 647N acid6456631500000.6510.060.05
ATTO 594 acid6026211200000.850.260.51
ATTO 514 acid510531115,0000.850.210.08
ATTO 565 acid5625891200000.900.270.12
ATTO 390 acid39047524000.900.460.09
ATTO 425 acid438484450000.900.190.17
ATTO 495 acid497525800000.20.450.37
ATTO 550 acid5535741200000.800.230.10
ATTO 590 DBCO5926211200000.800.390.43
ATTO 590 TCO5926211200000.800.390.43
ATTO 590 Tetrazine5926211200000.800.390.43
ATTO 610 acid6156321500000.700.030.06
ATTO 620 acid61964112000010.510.040.06
ATTO 633 acid6296511300000.6410.040.05
ATTO 655 acid6616791250000.310.240.08
ATTO 680 acid6796961250000.300.300.17
ATTO 700 acid6997151200000.250.260.41
Show More (11)

References

View all 17 references: Citation Explorer
Closing the green gap of photosystem I with synthetic fluorophores for enhanced photocurrent generation in photobiocathodes.
Authors: Morlock, Sascha and Subramanian, Senthil K and Zouni, Athina and Lisdat, Fred
Journal: Chemical science (2023): 1696-1708
A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores.
Authors: Steinmetzger, Christian and Palanisamy, Navaneethan and Gore, Kiran R and Höbartner, Claudia
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2019): 1931-1935
Diffusional Dynamics of Tetraalkylphosphonium Ionic Liquid Films Measured by Fluorescence Correlation Spectroscopy.
Authors: Mendivelso-Pérez, Deyny L and Farooq, Muhammad Qamar and Santra, Kalyan and Anderson, Jared L and Petrich, Jacob W and Smith, Emily A
Journal: The journal of physical chemistry. B (2019): 4943-4949
Site-selective functionalization of plasmonic nanopores for enhanced fluorescence emission rate and Förster resonance energy transfer.
Authors: Zambrana-Puyalto, Xavier and Maccaferri, Nicolò and Ponzellini, Paolo and Giovannini, Giorgia and De Angelis, Francesco and Garoli, Denis
Journal: Nanoscale advances (2019): 2454-2461
A CE-FL based method for real-time detection of in-capillary self-assembly of the nanoconjugates of polycysteine ligand and quantum dots.
Authors: Wang, Jianhao and Zhu, Zhilan and Qiu, Lin and Wang, Jianpeng and Wang, Xiang and Xiao, Qicai and Xia, Jiang and Liu, Li and Liu, Xiaoqian and Feng, Wei and Wang, Jinmei and Miao, Peng and Gao, Liqian
Journal: Nanotechnology (2018): 274001
Page updated on November 23, 2024

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Catalog Number70240
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Physical properties

Molecular weight

691.17

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.39

Correction Factor (280 nm)

0.43

Extinction coefficient (cm -1 M -1)

120000

Excitation (nm)

592

Emission (nm)

621

Quantum yield

0.80

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Gallery Image 1
With EDAC or other equivalent activating coupling agents, fluorescent dyes, such as ATTO 590 acid, can react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.