ATTO 590 Tetrazine

• Ex/Em: 592/621 nm
• Extinction coefficient: 120,000 cm^-1M^-1
• Reactive group: Tetrazine
• Click Chemistry Conjugation: Provides high yield and rapid, copper-free labeling of TCO-modified biomolecules
• High Quantum Yield & Stability: Delivers bright fluorescence with high photostability and thermal resilience
• Compatibility with Quenchers: Fluorescence effectively quenched by BHQ®-2 dye, enabling precise FRET applications and molecular beacon assays

ATTO 590 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent photostability and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 575-610 nm wavelength range. ATTO 590 emits in the orange-red region of the visible spectrum, with fluorescence effectively quenched by BHQ®-2 dye. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. ATTO 590 is a suitable alternative to Alexa Fluor® 594 for these applications.

ATTO 590 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.