ATTO 488 Tetrazine

• Ex/Em: 499/520 nm
• Extinction coefficient: 90,000 cm^-1M^-1
• Reactive group: Tetrazine
• Click Chemistry Conjugation: Provides rapid and copper-free labeling of TCO-modified biomolecules
• Superior Brightness & Stability: Delivers robust, steady fluorescence for sensitive applications
• High Hydrophilicity: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

ATTO 488 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.