ATTO 488 azide

• Ex/Em: 499/520 nm
• Extinction coefficient: 90,000 cm^-1M^-1
• Reactive group: azide
• Efficient Conjugation: Readily and selectively labels alkyne targets via click chemistry
• High Quantum Yield & Photostability: Delivers consistent, intense fluorescence under demanding conditions
• High Hydrophilicity: Efficient aqueous dispersion enhances signal clarity for advanced imaging and live-cell applications

ATTO 488 is a hydrophilic, rhodamine-based fluorescent dye with exceptional water solubility. It is characterized by strong absorption, a high fluorescence quantum yield, and exceptional photostability, making it highly suitable for advanced fluorescence imaging techniques. The dye exhibits optimal excitation within the 480-515 nm wavelength range, aligning precisely with the 488 nm emission line of the Argon-Ion laser. ATTO 488 is particularly effective for single-molecule detection and super-resolution microscopy methods such as PALM, dSTORM, and STED. Moreover, it is well-suited for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other bioanalytical applications.

The azide derivative of ATTO 488 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.