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ATTO 488 azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 657.67 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.10 |
| Extinction coefficient (cm -1 M -1) | 90000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.80 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| ATTO 488 maleimide |
| ATTO 488 acid |
| ATTO 488 alkyne |
| Overview |  SDSProtocol |
Molecular weight 657.67 | Correction Factor (260 nm) 0.25 | Correction Factor (280 nm) 0.10 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.80 |
ATTO 488 is a rhodamine-based fluorescent probe characterized by its excellent aqueous solubility and excellent spectral charactistics. It exhibits strong absorption, high fluorescence quantum yield, and outstanding photostability, making it particularly suitable for fluorescence imaging applications. ATTO 488 is highly effective for single-molecule detection and advanced high-resolution microscopy techniques such as PALM, dSTORM, and STED. Moreover, it is applicable in flow cytometry (FACS), fluorescence in-situ hybridization (FISH), and various other biological assays. The azide derivative of ATTO 488 can undergo a copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) with terminal alkynes to form 1,2,3-triazoles. It can also participate in Staudinger ligation with phosphine-containing molecules. Additionally, the azide group is reactive towards cyclooctynes and BCN moieties via strain-promoted azide-alkyne cycloaddition (SPAAC), facilitating copper-free bioorthogonal labeling reactions. These versatile reactivity profiles enhance the utility of ATTO 488 in various biochemical and molecular biology applications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ATTO 488 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 152.052 µL | 760.26 µL | 1.521 mL | 7.603 mL | 15.205 mL |
| 5 mM | 30.41 µL | 152.052 µL | 304.104 µL | 1.521 mL | 3.041 mL |
| 10 mM | 15.205 µL | 76.026 µL | 152.052 µL | 760.26 µL | 1.521 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.10 |
| Extinction coefficient (cm -1 M -1) | 90000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.80 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 488 azide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| ATTO 488 DBCO | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
Images
Figure 1. ATTO 488 azide
References
View all 32 references: Citation Explorer
Time-resolved fluorescence anisotropy with Atto 488-labeled phytochrome Agp1 from Agrobacterium fabrum.
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Authors: Elkurdi, Afaf and Guigas, Gernot and Hourani-Alsharafat, Latifa and Scheerer, Patrick and Nienhaus, Gerd Ulrich and Krauß, Norbert and Lamparter, Tilman
Journal: Photochemistry and photobiology (2024): 561-572
Analysis of photon trajectories from diffusing single molecules.
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Authors: Gopich, Irina V and Kim, Jae-Yeol and Chung, Hoi Sung
Journal: The Journal of chemical physics (2023)
Curvature preference of cubic CsPbBr3 quantum dots embedded onto phospholipid bilayer membranes.
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Authors: Chairil, Ricki and Malmstadt, Noah
Journal: Soft matter (2023): 3966-3974
Interactions of the Kv1.1 Channel with Peptide Pore Blockers: A Fluorescent Analysis on Mammalian Cells.
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Authors: Orlov, Nikita A and Kryukova, Elena V and Efremenko, Anastasia V and Yakimov, Sergey A and Toporova, Victoria A and Kirpichnikov, Mikhail P and Nekrasova, Oksana V and Feofanov, Alexey V
Journal: Membranes (2023)
Spatial distribution of single guest molecules along thickness of thin films of poly(2-hydroxyethyl acrylate).
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Authors: Ito, Syoji and Hiratsuka, Kengo and Takei, Satoshi and Nishi, Hiroyasu and Kitagawa, Daichi and Kobatake, Seiya and Miyasaka, Hiroshi
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 175-184
Atto488-Agitoxin 2-A Fluorescent Ligand with Increased Selectivity for Kv1.3 Channel Binding Site.
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)
Authors: Denisova, Kristina R and Orlov, Nikita A and Yakimov, Sergey A and Kirpichnikov, Mikhail P and Feofanov, Alexey V and Nekrasova, Oksana V
Journal: Bioengineering (Basel, Switzerland) (2022)
Lipophilic probe behavior in microemulsions evaluated by fluorescence correlation spectroscopy.
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
Authors: Yamamoto, Johtaro and Suzuki, Yoshio and Ogawa, Yoshikatsu and Kamata, Tomoyuki and Hashimoto, Hinako and Kunitake, Masashi and Kato, Dai
Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry (2022): 401-408
A Model of F-actin Organization in Granuloreticulopodia in Foraminifera: Morphogenetic and Evolutionary Implications from Novel Fluorescent and Polarised Light Observations.
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Authors: Goleń, Jan and Tyszka, Jarosław and Godos, Karolina and Janse, Max
Journal: Protist (2022): 125886
Evaluation of [Cys(ATTO 488)8]Dermorphin-NH2 as a novel tool for the study of μ-opioid peptide receptors.
Authors: Giakomidi, Despina and Bird, Mark F and McDonald, John and Marzola, Erika and Guerrini, Remo and Chanoch, Serena and Sabu, Nidhuna and Horley, Barbara and Calo, Girolamo and Lambert, David G
Journal: PloS one (2021): e0250011
Authors: Giakomidi, Despina and Bird, Mark F and McDonald, John and Marzola, Erika and Guerrini, Remo and Chanoch, Serena and Sabu, Nidhuna and Horley, Barbara and Calo, Girolamo and Lambert, David G
Journal: PloS one (2021): e0250011
Multimodal fluorescently labeled polymer-coated GdF3 nanoparticles inhibit degranulation in mast cells.
Authors: Shapoval, Oleksandr and Sulimenko, Vadym and Klebanovych, Anastasiya and Rabyk, Mariia and Shapoval, Pavlo and Kaman, Ondřej and Rydvalová, Eliška and Filipová, Marcela and Dráberová, Eduarda and Dráber, Pavel and Horák, Daniel
Journal: Nanoscale (2021): 19023-19037
Authors: Shapoval, Oleksandr and Sulimenko, Vadym and Klebanovych, Anastasiya and Rabyk, Mariia and Shapoval, Pavlo and Kaman, Ondřej and Rydvalová, Eliška and Filipová, Marcela and Dráberová, Eduarda and Dráber, Pavel and Horák, Daniel
Journal: Nanoscale (2021): 19023-19037