ATTO 390 azide

• Ex/Em: 390/475 nm
• Extinction coefficient: 24,000 cm^-1M^-1
• Reactive group: azide
• Precise Conjugation: Readily and selectively labels alkyne targets via click chemistry
• High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications.
• Large Stokes Shift: Enables effective signal separation, reducing background interference

ATTO 390 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, good photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited within the 360-410 nm range, with a mercury arc lamp (emission lines at 365 nm and 405 nm) serving as an effective excitation source. This dye is well-suited for applications in single-molecule detection and advanced high-resolution microscopy techniques, including PALM, dSTORM, and STED. Additionally, ATTO 390 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other diverse biological assays.

The azide derivative of ATTO 390 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.