ATTO 495 azide

• Ex/Em: 497/525 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Reactive group: azide
• Precise Conjugation: Readily and selectively labels alkyne targets via click chemistry
• Superior Brightness & Stability: Delivers intense fluorescence with high photostability and thermal stability
• Long-Lived Phosphorescence: Enables sustained emission in solid matrices or at low temperatures, ideal for time-resolved spectroscopy

ATTO 495 is a green fluorescent dye derived from acridine orange, known for its strong absorption, high fluorescence quantum yield, excellent photostability, and superior thermal stability. It exhibits moderate hydrophilicity and is highly soluble in polar solvents such as DMF and DMSO, with an optimal excitation range of 465-510 nm. Notably, ATTO 495 exhibits intense and long-lived phosphorescence in solid matrices or at low temperatures. This dye is well-suited for advanced applications in single-molecule detection and high-resolution microscopy techniques, such as PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a wide range of other biological assays.

The azide derivative of ATTO 495 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.