ATTO 495 Tetrazine

• Ex/Em: 497/525 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Reactive group: Tetrazine
• Click Chemistry Conjugation: Provides rapid and copper-free labeling of TCO-modified biomolecules with high yield
• Superior Brightness & Stability: Delivers intense fluorescence with high photostability and thermal stability
• Long-Lived Phosphorescence: Enables sustained emission in solid matrices or at low temperatures, ideal for time-resolved spectroscopy

ATTO 495 is a green fluorescent dye derived from acridine orange, known for its strong absorption, high fluorescence quantum yield, excellent photostability, and superior thermal stability. It exhibits moderate hydrophilicity and is highly soluble in polar solvents such as DMF and DMSO, with an optimal excitation range of 465-510 nm. Notably, ATTO 495 exhibits intense and long-lived phosphorescence in solid matrices or at low temperatures. This dye is well-suited for advanced applications in single-molecule detection and high-resolution microscopy techniques, such as PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a wide range of other biological assays.

ATTO 495 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.