ATTO 495 amine

• Ex/Em: 497/525 nm
• Extinction coefficient: 80,000 cm^-1M^-1
• Reactive group: amine
• Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
• Superior Brightness & Stability: Delivers intense fluorescence with high photostability and thermal stability
• Long-Lived Phosphorescence: Enables sustained emission in solid matrices or at low temperatures, ideal for time-resolved spectroscopy

ATTO 495 is a green fluorescent dye derived from acridine orange, known for its strong absorption, high fluorescence quantum yield, excellent photostability, and superior thermal stability. It exhibits moderate hydrophilicity and is highly soluble in polar solvents such as DMF and DMSO, with an optimal excitation range of 465-510 nm. Notably, ATTO 495 exhibits intense and long-lived phosphorescence in solid matrices or at low temperatures. This dye is well-suited for advanced applications in single-molecule detection and high-resolution microscopy techniques, such as PALM, dSTORM, and STED microscopy. It is also compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and a wide range of other biological assays.

ATTO 495 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.