ATTO 390 amine

• Ex/Em: 390/475 nm
• Extinction coefficient: 24,000 cm^-1M^-1
• Reactive group: amine
• Versatile Conjugation: Efficiently labels carbonyl groups (e.g., aldehyde, ketone, and carboxy groups); also an amine donor for enzymatic transamination labeling
• High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
• Large Stokes Shift: Enables effective signal separation, reducing background interference

ATTO 390 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, good photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited within the 360-410 nm range, with a mercury arc lamp (emission lines at 365 nm and 405 nm) serving as an effective excitation source. This dye is well-suited for applications in single-molecule detection and advanced high-resolution microscopy techniques, including PALM, dSTORM, and STED. Additionally, ATTO 390 is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), and other diverse biological assays.

ATTO 390 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.