ATTO 425 amine

• Ex/Em: 438/484 nm
• Extinction coefficient: 45,000 cm^-1M^-1
• Reactive group: amine
• Versatile Conjugation: Efficiently labels carbonyl groups (e.g., aldehyde, ketone, and carboxy groups); also an amine donor for enzymatic transamination labeling
• High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
• Enhanced Signal Separation: Minimal excitation and emission overlap reduces background interference

ATTO 425 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, excellent photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited in the 405-455 nm wavelength range. These properties make ATTO 425 particularly suitable for applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. Additionally, ATTO 425 is well-suited for use in flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 425 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.