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ATTO 425 amine

ATTO 425 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, excellent photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited in the 405-455 nm wavelength range. These properties make ATTO 425 particularly suitable for applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. Additionally, ATTO 425 is well-suited for use in flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

ATTO 425 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
ATTO 390 amine39047524000.900.460.09
ATTO 425 DBCO438484450000.900.190.17
ATTO 425 TCO438484450000.900.190.17
ATTO 425 Tetrazine438484450000.900.190.17
ATTO 495 amine497525800000.20.450.37

References

View all 4 references: Citation Explorer
Ratiometric Fluorescent Biosensor Based on Self-Assembled Fluorescent Gold Nanoparticles and Duplex-Specific Nuclease-Assisted Signal Amplification for Sensitive Detection of Exosomal miRNA.
Authors: Sun, Zhiwei and Li, Juan and Yang, Yufei and Tong, Yao and Li, Hui and Wang, Chuanxin and Du, Lutao and Jiang, Yanyan
Journal: Bioconjugate chemistry (2022): 1698-1706
Ratiometric Fluorescence Detection of Colorectal Cancer-Associated Exosomal miR-92a-3p with DSN-Assisted Signal Amplification by a MWCNTs@Au NCs Nanoplatform.
Authors: Sun, Zhiwei and Li, Juan and Tong, Yao and Zhao, Li and Zhou, Xiaoyu and Li, Hui and Wang, Chuanxin and Du, Lutao and Jiang, Yanyan
Journal: Biosensors (2022)
Avidin/Biotin Bioinspired Platform for Dual In Vivo 18F-PET/NIRF Molecular Imaging.
Authors: Damont, Annelaure and Boisgard, Raphael and Dollé, Frédéric and Hollocou, Morgane and Kuhnast, Bertrand
Journal: Bioconjugate chemistry (2017): 2524-2529
Fluorescence of dyes in solutions with high absorbance. Inner filter effect correction.
Authors: Fonin, Alexander V and Sulatskaya, Anna I and Kuznetsova, Irina M and Turoverov, Konstantin K
Journal: PloS one (2014): e103878
Page updated on October 27, 2024

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Catalog Number70218
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Physical properties

Molecular weight

557.57

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.19

Correction Factor (280 nm)

0.17

Extinction coefficient (cm -1 M -1)

45000

Excitation (nm)

438

Emission (nm)

484

Quantum yield

0.90

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200