ATTO 425 azide

ATTO 425 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, excellent photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited in the 405-455 nm wavelength range. These properties make ATTO 425 particularly suitable for applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. Additionally, ATTO 425 is well-suited for use in flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

The azide derivative of ATTO 425 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.