ATTO 590 azide

• Ex/Em: 592/621 nm
• Extinction coefficient: 120,000 cm^-1M^-1
• Reactive group: azide
• Click Chemistry Conjugation: Efficiently and rapidly labels terminal alkynes via CuAAC and copper-free SPAAC
• High Quantum Yield & Stability: Provides bright fluorescence with high photostability and thermal resilience
• Compatibility with Quenchers: Fluorescence effectively quenched by BHQ®-2 dye, enabling precise FRET applications and molecular beacon assays

ATTO 590 is a rhodamine-based fluorescent dye characterized by its strong absorption, high fluorescence quantum yield, and excellent photostability and thermal stability. It exhibits moderate hydrophilicity and is optimally excited within the 575-610 nm wavelength range. ATTO 590 emits in the orange-red region of the visible spectrum, with fluorescence effectively quenched by BHQ®-2 dye. This dye is particularly suited for advanced applications in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED microscopy. Additionally, it is compatible with flow cytometry (FACS), fluorescence in situ hybridization (FISH), FRET, and various other biological assays. ATTO 590 is a suitable alternative to Alexa Fluor® 594 for these applications.

The azide derivative of ATTO 590 is widely used for labeling terminal alkynes on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with alkyne-containing molecules and strain-promoted alkyne-azide cycloaddition (SPAAC) with DBCO- or BCN-containing molecules.