ATTO 425 alkyne

• Ex/Em: 438/484 nm
• Extinction coefficient: 45,000 cm^-1M^-1
• Reactive group: alkyne
• Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
• High Quantum Yield & Photostability: Delivers strong, stable fluorescence for sensitive applications
• Enhanced Signal Separation: Minimal excitation and emission overlap reduces background interference

ATTO 425 is a coumarin-based fluorescent dye characterized by its high fluorescence quantum yield, large Stokes shift, excellent photostability, and low molecular weight. It exhibits moderate hydrophilicity and is optimally excited in the 405-455 nm wavelength range. These properties make ATTO 425 particularly suitable for applications in single-molecule detection and high-resolution microscopy techniques, including PALM, dSTORM, and STED microscopy. Additionally, ATTO 425 is well-suited for use in flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays.

The alkyne derivative of ATTO 425 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.