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ATTO 488 alkyne
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 626.66 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.10 |
| Extinction coefficient (cm -1 M -1) | 90000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.80 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Alternative formats
| ATTO 488 maleimide |
| ATTO 488 acid |
| ATTO 488 azide |
| Overview |  SDSProtocol |
Molecular weight 626.66 | Correction Factor (260 nm) 0.25 | Correction Factor (280 nm) 0.10 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 499 | Emission (nm) 520 | Quantum yield 0.80 |
ATTO 488 is a rhodamine-based fluorescent probe with excellent aqueous solubility and favorable spectral properties. It exhibits strong absorption, high fluorescence quantum yield, and notable photostability, making it optimal for fluorescence imaging applications. ATTO 488 is particularly effective in single-molecule detection and high-resolution microscopy techniques such as PALM, dSTORM, and STED. It is also suitable for flow cytometry (FACS), fluorescence in situ hybridization (FISH), and various other biological assays. The alkyne derivative of ATTO 488 can participate in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) with terminal azides, forming a stable triazole linker. Additionally, ATTO 488 alkyne can function as a bio-orthogonal or biologically unique hapten for applications involving signal amplification.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of ATTO 488 alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 159.576 µL | 797.881 µL | 1.596 mL | 7.979 mL | 15.958 mL |
| 5 mM | 31.915 µL | 159.576 µL | 319.152 µL | 1.596 mL | 3.192 mL |
| 10 mM | 15.958 µL | 79.788 µL | 159.576 µL | 797.881 µL | 1.596 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.25 |
| Correction Factor (280 nm) | 0.10 |
| Extinction coefficient (cm -1 M -1) | 90000 |
| Excitation (nm) | 499 |
| Emission (nm) | 520 |
| Quantum yield | 0.80 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| iFluor® 488 alkyne | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
| ATTO 488 DBCO | 499 | 520 | 90000 | 0.80 | 0.25 | 0.10 |
Images
Figure 1. ATTO 488 alkyne
References
View all 7 references: Citation Explorer
The new live imagers MitoMM1/2 for mitochondrial visualization.
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
Authors: Maeda, Miwa and Suzuki, Mayu and Takashima, Shigeo and Sasaki, Tsutomu and Oh-Hashi, Kentaro and Takemori, Hiroshi
Journal: Biochemical and biophysical research communications (2021): 50-54
DNA-templated control of chirality and efficient energy transport in supramolecular DNA architectures with aggregation-induced emission.
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Authors: Ucar, Hülya and Wagenknecht, Hans-Achim
Journal: Chemical science (2021): 10048-10053
Endoplasmic reticulum phospholipid scramblase activity revealed after protein reconstitution into giant unilamellar vesicles containing a photostable lipid reporter.
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Authors: Mathiassen, Patricia P M and Menon, Anant K and Pomorski, Thomas Günther
Journal: Scientific reports (2021): 14364
Importance of probe design for bioanalysis of oligonucleotides using hybridization-based LC-fluorescence assays.
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
Authors: Ji, Yuhuan and Liu, Yijiang and Xia, Wanhong and Behling, Alexander and Meng, Min and Bennett, Patrick and Wang, Laixin
Journal: Bioanalysis (2019): 1917-1925
The effect of local dynamics of Atto 390-labeled lysozyme on fluorescence anisotropy modeling.
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
Authors: Babcock, Jeremiah J and Brancaleon, Lorenzo
Journal: Biopolymers (2015): 285-95
Binding assay for low molecular weight analytes based on reflectometry of absorbing molecules in porous substrates.
Authors: Stephan, Milena and Kramer, Corinna and Steinem, Claudia and Janshoff, Andreas
Journal: The Analyst (2014): 1987-92
Authors: Stephan, Milena and Kramer, Corinna and Steinem, Claudia and Janshoff, Andreas
Journal: The Analyst (2014): 1987-92
Fluorescently labeled substrates for monitoring α1,3-fucosyltransferase IX activity.
Authors: Lunau, Nathalie and Seelhorst, Katrin and Kahl, Stefanie and Tscherch, Kathrin and Stacke, Christina and Rohn, Sascha and Thiem, Joachim and Hahn, Ulrich and Meier, Chris
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2013): 17379-90
Authors: Lunau, Nathalie and Seelhorst, Katrin and Kahl, Stefanie and Tscherch, Kathrin and Stacke, Christina and Rohn, Sascha and Thiem, Joachim and Hahn, Ulrich and Meier, Chris
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2013): 17379-90